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Apoptosis and tumour cell death in response to pro-apoptotic gene


Nik Abd Rahman, Nik Mohd Afizan and Allaudin, Zeenathul Nazariah and Mohamed Mustapha, Noordin and Ismail, Ruzila and Mustafa, Nor Hidayah and Mohd Lila, Mohd Azmi (2011) Apoptosis and tumour cell death in response to pro-apoptotic gene. Pertanika Journal of Tropical Agricultural Science, 34 (1). pp. 163-166. ISSN 1511-3701; ESSN: 2231-8542


The process of cell death, or apoptosis, is commonly defined by its distinct morphological characteristic. Apoptosis is considered a vital component of various processes including normal cell turnover, proper development and functioning of the immune system, the cellular antiviral response pathway, embryonic development and chemical-induced cell death. In this study, retroviral vector was utilized as the gene delivery vehicles and the tumour-specific viral death effector VP3 as pro-apoptotic agent for malignant colon cancer cells treatment. Here, pro-apoptotic gene inducing apoptosis in CT26 colon tumour cells is reported. In addition, the activation of a typical apoptotic programme was observed in response to this therapeutic agent. An increased number of colon tumour cells suffered apoptosis upon treatment with pro-apoptotic agent. As a result, these tumour cells displayed loss of membrane asymmetry and impermeability, cell shrinkage, nuclear condensation and DNA fragmentation under H&E images. In the presence of VP3, TEM also confirmed that VP3-treated CT26 tumour cells showed apoptotic features. These results suggested that VP3 was a potential pro-apoptotic agent and the apoptosis induced by VP3 was a key anti-tumour mechanism.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Institute of Bioscience
Publisher: Universiti Putra Malaysia Press
Keywords: CT26; Mouse models; Pro-apoptotic agent; Retroviral vector; Tumour; VP3
Depositing User: Azizan Arshad
Date Deposited: 09 Dec 2013 07:10
Last Modified: 30 Oct 2019 06:35
URI: http://psasir.upm.edu.my/id/eprint/13215
Statistic Details: View Download Statistic

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