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Apoptosis and cell cycle arrest of human colorectal cancer cell line HT-29 induced by vanillin.


Ho, Ket Li and Saiful Yazan, Latifah and Ismail, Norsharina and Ismail, Maznah (2009) Apoptosis and cell cycle arrest of human colorectal cancer cell line HT-29 induced by vanillin. Cancer Epidemiology, 33 (2). pp. 155-160. ISSN 1877-7821; ESSN: 1877-783X


Background: Vanillin is responsible for the flavor and smell of vanilla, a widely used flavoring agent. Previous studies showed that vanillin could enhance the repair of mutations and thus function as an anti-mutagen. However, its role in cancer, a disease that is closely related to mutation has not yet been fully elucidated. Methods: Hence, this study investigated the cytolytic and cytostatic properties of vanillin against HT-29, a human colorectal cancer cell line. Methods used including cell viability assay, acridine orange (AO)–ethidium bromide (EB) double staining cell morphological analysis, Cell cycle analysis, annexin V–propidium iodide apoptosis test and 5-bromo-2-deoxyuridine (BrdU)-labeling cell proliferation assay. Results: Results showed that apoptosis was induced by vanillin and the IC50 for HT-29 and NIH/3T3 normal cell lines were 400 μg/ml and 1000 μg/ml, respectively. Different concentrations of vanillin arrest cell cycle at different checkpoints. 5-Bromo-2-deoxyuridine-labeling cell proliferation assay showed that G0/G1 arrest was achieved at lower concentration of vanillin (200 μg/ml) while cell cycle analysis by flow cytometer showed that G2/M arrest occurs at higher concentration of vanillin (1000 μg/ml). Conclusion: Cytolytic and cytostatic effects shown by vanillin showed that it could be a useful colorectal cancer preventive agent. Further in vivo study should be carried out to confirm that similar effects could happen in animals.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Institute of Bioscience
DOI Number: https://doi.org/10.1016/j.canep.2009.06.003
Publisher: Elsevier
Keywords: Cell viability; MTS assay; Cell morphology; Acridine orange–ethidium bromide; Cell cycle; Apoptosis; Annexin V–propidium iodide; 5-Bromo-2-deoxyuridine (BrdU); Anti-proliferation
Depositing User: Raja Norazlinda Raja Azenam
Date Deposited: 04 Dec 2014 06:53
Last Modified: 15 Sep 2015 04:01
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.canep.2009.06.003
URI: http://psasir.upm.edu.my/id/eprint/13214
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