UPM Institutional Repository

Antinociceptive and anti-inflammatory effects of stachytarpheta jamaicensis (L.) Vahl (Verbenaceae)in experimental animal models.


Citation

Sulaiman, Mohd Roslan and Zakaria, Zainul Amiruddin and Chiong, Hoe Siong and Lai, Sook Kean and Israf Ali, Daud Ahmad and Tengku Mohamad, Tengku Azam Shah (2009) Antinociceptive and anti-inflammatory effects of stachytarpheta jamaicensis (L.) Vahl (Verbenaceae)in experimental animal models. Medical Principles and Practice, 18 (4). pp. 272-279. ISSN 1011-7571; ESSN: 1423-0151

Abstract

Objective: The present study was carried out to explore the antinociceptive as well as the anti-inflammatory effects of an ethanol extract of Stachytarpheta jamaicensis (L.) Vahl (EESJ) using 3 models of nociception and 2 models of inflammation in experimental animals. Materials and Methods: EESJ was prepared by overnight soaking of the oven-dried (50°C; 72 h) ground leaves (500 g) in 80% ethanol (1:5; w/v). The filtrate was evaporated to dryness (50°C), resuspended in distilled water at concentrations to provide the desired doses of 50, 100 and 150 mg/kg. For antinociceptive effects, 3 models were used: acetic acid-induced abdominal writhing, hot-plate- and formalin-induced paw-licking tests; for anti-inflammatory effects, 2 models were used - carrageenan-induced paw edema and cotton-pellet-induced granuloma tests. Appropriate doses were administered intraperitoneally (i.p.) to mice/rats prior to each test. The mechanisms of antinociceptive action of the extract were also investigated by pretreatment with naloxone (5 mg/kg, i.p.). Results: The extract exhibited significant (p < 0.05) antinociceptive activity in all nociceptive models tested with dose-dependent activity observed using the abdominal writhing and formalin tests. Pretreatment with naloxone partially, but significantly (p < 0.05) reversed the antinociceptive activity of the extract when assessed using the abdominal-writhing- and formalin-induced paw-licking tests, and completely inhibited its activity when the hot-plate test was used. The extract also showed significant (p < 0.05) anti-inflammatory activity in both the acute (carrageenan-induced paw edema test) and the chronic (cotton-pellet granuloma test) tests. Conclusion: This study showed the potential of EESJ to exert antinociceptive and anti-inflammatory activities, the former being modulated via peripheral and central mechanisms and involving, in part, activation of the opioid receptor system.


Download File

Full text not available from this repository.

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1159/000215723
Publisher: Karger
Keywords: Anti-inflammatory activity; Antinociceptive activity; Opioid mechanism; Stachytarpheta jamaicensis
Depositing User: Raja Norazlinda Raja Azenam
Date Deposited: 05 Dec 2014 09:12
Last Modified: 05 Dec 2014 09:12
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1159/000215723
URI: http://psasir.upm.edu.my/id/eprint/13170
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item