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Anti-inflammatory effect of Mitragyna speciosa crude methanol extract on the guinea pig ileum.


Shaik Mossadeq, Wan Mastura and Khalid, Syamimi and M.P, Azyyati and Z.A., Zakaria and Abdul Kadir, Arifah and Rajion, Mohamed Ali and M Taufik, Hidayat and M.L., Jabit and M. R., Sulaiman (2009) Anti-inflammatory effect of Mitragyna speciosa crude methanol extract on the guinea pig ileum. Planta Medica, 75 (9). 1012-1012 . ISSN 0032-0943; ESSN: 1439-0221


In a recent study, we have shown that the crude methanol extract of Mitragyna speciosa (of the family Rubiaceae) exerted significant in vivo anti-inflammatory activities in rodents as evident in the carageenan-induced paw edema and cotton pellet-induced granuloma tests [1], but the underlying mechanism is poorly understood. Our present study aims to explore the anti-inflammatory activities of this plant in vitro in order to rationalize the traditional use of this plant in the treatment of some stomach ailments [2]. The pharmacologic actions of M. speciosa were assessed by measuring the mechanical activity of isolated guinea pig ileum strips in an organ bath. The resultant methanol extract (0.01–0.05mg/ml) caused a stimulatory effect followed by a relaxation of ileal activities at a higher dose (0.3–5mg/ml) (p<0.05). These results indicate that M. speciosa exert spasmogenic effects at a lower dose and a spasmolytic effect at a higher dose thus corroborating the use of plant in the treatment of diarrhoea and constipation. Moreover, results indicated that pretreatment with M. speciosa (0.3–5mg/ml) which were tested positive for flavonoids, alkaloids, saponins, sterols and tannins produced significant concentration-dependant inhibition of spasmogenic activities when exposed to single submaximal contraction induced by histamine (H) and bradykinin (B). These results suggest that the anti-inflammatory activity of M. speciosa is mediated possibly through the H and B receptor antagonism, thus providing a scientific basis for the folkloric use of this plant in stomach disorders.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.1055/s-0029-1234736
Publisher: Georg Thieme Verlag
Keywords: Anti-inflammatory, Mitragyna speciosa; Guinea pig ileum.
Depositing User: Azizan Arshad
Date Deposited: 09 Dec 2013 07:38
Last Modified: 09 Dec 2013 07:38
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1055/s-0029-1234736
URI: http://psasir.upm.edu.my/id/eprint/13153
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