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Amelioration of mangosteen micro propagation through leaf and seed segments (Garcinia Mangostana L.)


Sirchi, Mohammad Hossein Torabi and Abdul Kadir, Mihdzar and Abdul Aziz, Maheran and Abdul Rashid, Azmi and Rafat, Arash and Javadi, Mohammad Bagher (2008) Amelioration of mangosteen micro propagation through leaf and seed segments (Garcinia Mangostana L.). African Journal of Biotechnology, 7 (12). art. no. 5BED1AE7507. pp. 2025-2029. ISSN 1684–5315


A micro-propagation system was developed for mangosteen (Garcinia Mangostana L.) using leaf sections and mature seeds. This study investigated the effect of various modified Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurin (BAP) alone or in combination with α-naphthalene acetic acid (NAA) on multiple shoot induction from leaf and half seed explants. Among the treatments, leaf explants on medium supplemented with 2.0 mgL־¹ (w/v) of BAP gave the highest percentage of shoot formation (70.07%), highest number of shoots formed per explant (46.5%) and showed high mean shoot height (0.48 cm). It was observed that medium with 0.5 mgL־¹ NAA, did not induce shoot organogenesis, but induced callus in 90% of the leaf explants. Seed explantsgave the highest percentage of shoot formation (60.2%) and mean number of shoots per explant (9.5) on medium supplemented with 20 mgL־¹ (w/v) BAP and 10 mgL־¹ (w/v) NAA. However, highest mean shoot height (0.69 cm) was observed on medium supplemented with 10 mgL־¹ (w/v) BAP and 10 mgL־¹ (w/v) NAA. Callus induction from seeds was also observed in most of the treatments. Medium-supplemented 20 mgL־¹ (w/v) NAA gave the highest percentage of callus formation (83.36%). Subsequently, roots were induced from the shoots by pretreating these shoots in various indol-3-butyric acid (IBA) and NAA concentrations in various MS salt strengths. Root formation was obtained on one-quarter strength MS salt medium supplemented with 0.1 mg/L NAA (90.4%). Plantlets were acclimatized on media composed of soil + sand+ organic matter+ vermiculite (2: 2: 1: 1) with 80% survival.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
DOI Number: https://doi.org/10.5897/AJB2008.000-5051
Publisher: Academic Journals
Keywords: Garcinia mangostana L.; 6-benzylaminopurine; α-napthalene acetic acid; Indole-3-butyric acid
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 06 Jun 2015 08:30
Last Modified: 03 May 2016 09:26
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5897/AJB2008.000-5051
URI: http://psasir.upm.edu.my/id/eprint/12999
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