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Antibacterial and sporicidal activity of Eugenia polyantha Wight against Bacillus cereus and Bacillus subtilis


Citation

Lau, Kah Yan and Mohd Zainin, Nurul Syazwani and Abas, Faridah and Rukayadi, Yaya (2014) Antibacterial and sporicidal activity of Eugenia polyantha Wight against Bacillus cereus and Bacillus subtilis. International Journal of Current Microbiology and Applied Sciences, 3 (12). pp. 499-510. ISSN 2319-7692; ESSN: 2319-7706

Abstract

The spore-forming bacteria, Bacillus spp., have often been associated with the contamination of rice and other starchy products. In this study, the extract of Indonesian bay leaf (Eugenia polyantha) was assessed for its antibacterial and sporicidal activities against vegetative cells and spores of Bacillus cereus ATCC33019 as well as Bacillus subtilis ATCC6633. The Minimum Inhibitory Concentration of E. polyantha extract against vegetative cells of B. cereus and B. subtilis were 0.31 and 0.63 mg/mL, respectively, while both their Minimum Bactericidal Concentration were 2.50 mg/mL. Time kill curves demonstrated that the bactericidal endpoint for B. cereus and B. subtilis was reached at concentration of 2.50 and 5.00 mg/mL, respectively (8× MIC) after 4 h of incubation. E. polyantha extract inactivated more than 3-log (99.99%) of B. cereus and B. subtilis spores at a concentration of 10.00 mg/mL (1.0%) after 1 h of incubation and the spores was completely killed at 25.00 mg/mL (2.5%). The sporicidal activity of E. polyantha extract was not affected by different temperatures treatment and alteration of pH. In conclusion, the potential anti-Bacillus activities of E. polyantha extract might support its use as a natural food preservative.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Institute of Bioscience
Publisher: Excellent Publishers
Keywords: Antibacterial; Sporicidal; Bacillus; Eugenia polyantha; Food preservative
Depositing User: Nabilah Mustapa
Date Deposited: 11 Jun 2018 08:35
Last Modified: 11 Jun 2018 08:35
URI: http://psasir.upm.edu.my/id/eprint/12929
Statistic Details: View Download Statistic

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