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A simple method to screen for azo-dye-degrading bacteria


Syed, Mohd Arif and Sim, H. K. and Khalid, A. and Abd. Shukor, Mohd. Yunus (2009) A simple method to screen for azo-dye-degrading bacteria. Journal of Environmental Biology, 30 (1). pp. 89-92. ISSN 0254-8704; ESSN: 2394-0379


A stab-culture method was adapted to screen for azo dyes-decolorizing bacteria from soil and water samples. Decolorized azo dye in the lower portion of the solid media indicates the presence of anaerobic azo dyes-decolorizing bacteria, while aerobic decolorizing bacteria decolorizes the surface portion of the solid media. Of twenty soil samples tested, one soil sample shows positive results for the decolourisation of two azo dyes; Biebrich scarlet (BS) and Direct blue 71 (DB) under anaerobic conditions. A gram negative and oxidase negative bacterial isolate was found to be the principal azo dyes degrader The isolate was identified by using the Biolog identification system as Serratia marcescens.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Publisher: Triveni Enterprises
Keywords: Azo dyes; Stab-culture; Screening method
Depositing User: Ms. Nida Hidayati Ghazali
Date Deposited: 02 Jul 2013 00:23
Last Modified: 27 Mar 2018 08:01
URI: http://psasir.upm.edu.my/id/eprint/12857
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