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Improved NARP-HPLC method for separating triglycerides of palm olein and its solid fractions obtained at low temperature storage


Citation

Phyo, Zaw Swe and Che Man, Yaakob and Mohd Ghazali, Hasanah (1996) Improved NARP-HPLC method for separating triglycerides of palm olein and its solid fractions obtained at low temperature storage. Food Chemistry, 56 (2). pp. 181-186. ISSN 0308-8146; ESSN: 1873-7072

Abstract / Synopsis

Non-aqueous reversed-phase high-performance liquid chromatography (NARP-HPLC) with refractive index (RI) detection is described and used for palm olein and its fractions obtained at 12.5 °C for 12–24 h. The calculation formula for fatty acid methyl esters (FAMEs) and carbon number (CN) from the data obtained by NARP-HPLC is described and correction factors for all carbon numbers and fatty acids are tabulated. The results were compared with those obtained from FAMEs analysed on 10% SP 2330 Supelco packed column gas-liquid chromatography (GLC) and CN analysed on 3% OV-1 Supelco packed column GLC. The results were found to agree well for C48, C50, C52, C16:0, C18:0 and C18:1 (correction factor ≈ 1.0); however, a slight variation was observed for components C54, C14:0 and C18:2 (correction factor 1.0 ± 0.37).


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Biotechnology
DOI Number: https://doi.org/10.1016/0308-8146(95)00178-6
Publisher: Elsevier
Keywords: Non-aqueous reversed-phase high-performance liquid chromatography (NARP-HPLC); Refractive index (RI); Palm olein
Depositing User: Nabilah Mustapa
Date Deposited: 25 Apr 2016 15:20
Last Modified: 25 Apr 2016 15:20
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/0308-8146(95)00178-6
URI: http://psasir.upm.edu.my/id/eprint/12755
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