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Extraction of useful DNA from different parts of the durian (Durio zibethinus) fruit


Citation

Ng, Wei Lun and Wong, Xue Jing and Ramaiya, Shiamala Devi and Sabran, Suzana and Hung, Bui Manh and Lee, Shiou Yih (2025) Extraction of useful DNA from different parts of the durian (Durio zibethinus) fruit. Pertanika Journal of Tropical Agricultural Science, 48 (4). art. no. 3. pp. 1109-1117. ISSN 2231-8542

Abstract

Successful deoxyribonucleic acid (DNA) extraction from the durian fruit allows effective molecular identification and genotyping of durian cultivars for quality assurance. While most genetic identification of durian samples used the leaf as the DNA source, studies exploring durian fruit as a source of DNA for molecular identification and genotyping are limited. In this study, four potential sources of genetic material: the peduncle, spine, carpel, and aril from the durian fruit were evaluated for DNA yield (in terms of concentration), quality (in terms of ratio of A260/A280), and suitability in PCR amplification (using genetic markers that target the chloroplast, nuclear, and microsatellite DNA regions). Results of this study show that the spine is a good potential source of genetic material alongside the peduncle.


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Additional Metadata

Item Type: Article
Subject: Agricultural Sciences
Subject: Genetics
Subject: Biotechnology
Divisions: Faculty of Agricultural Science and Forestry
DOI Number: https://doi.org/10.47836/pjtas.48.4.03
Publisher: Universiti Putra Malaysia
Keywords: Cultivar genotyping; DNA extraction; Fruit DNA; Genetic resources
Sustainable Development Goals (SDGs): SDG 2: Zero Hunger, SDG 9: Industry, Innovation and Infrastructure, SDG 15: Life on Land
Depositing User: Ms. Nur Faseha Mohd Kadim
Date Deposited: 15 Jul 2026 08:58
Last Modified: 15 Jul 2026 08:58
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.47836/pjtas.48.4.03
URI: http://psasir.upm.edu.my/id/eprint/127112
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