Citation
Abdul Rahman Omar
(2010)
Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method.
US:11/268,341.
Abstract
The present invention relates to a novel method to detect and differentiate different strains of infectious bursal disease virus (IBDV) in a chicken and other bird sample. RNA was obtained from said samples by using a pair of primer (Primer FVVC & RVVC) in a reverse transcriptase-polymerase chain reaction. Two different primer combinations (Primer IF & IVIR) and (Primer IF & RCLA) and real-time polymerase chain reaction conditions were designed and optimized for rapid differentiation of very virulent and vaccine strains of IBDV based on detection of signatory threshold cycle (Ct) and melting temperature (Tm) values.
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Additional Metadata
| Item Type: | Patent |
|---|---|
| Subject: | Veterinary Medicine |
| Subject: | Virology |
| Subject: | Molecular Biology |
| Application Number: | US:11/268,341 |
| Registration Number: | US:7659066 |
| Filing Date: | 2005-11-07 |
| Granted Date: | 2010-02-09 |
| Divisions: | Universiti Putra Malaysia |
| Keywords: | Infectious Bursal Disease Virus (IBDV); Chicken; Bird sample; Molecular biology; Detection; Differentiation; Strain typing; Reverse transcriptase-polymerase chain reaction (RT-PCR); Real-time PCR; Primer design |
| Sustainable Development Goals (SDGs): | SDG 3: Good Health and Well-being, SDG 2: Zero Hunger, SDG 12: Responsible Consumption and Production |
| Depositing User: | Ms. Nur Aina Ahmad Mustafa |
| Date Deposited: | 13 Jul 2026 02:44 |
| Last Modified: | 13 Jul 2026 02:44 |
| URI: | http://psasir.upm.edu.my/id/eprint/127048 |
| Statistic Details: | View Download Statistic |
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