Citation
Raja Noor Zaliha Abd. Rahman
(2007)
Method of crystallizing t1 lipase from geobacillus sp. strain t1.
PC:T/MY2007/000043.
Abstract
A method of crystallizing T1 lipase from Geobacillus sp. strain T1, wherein the method comprises the following steps: (a) Providing purified T1 lipase to be crystallized, (b) Preparing a crystallization mix comprising a buffer comprising 0.1 M NaH2PO4, 0.1 M KH2PO4, 2.0 M NaCl and 0.1 M MES pH 6.5, between 0.5 and 4.5 mg/mL of lipase from step (a), and between 1 and 2 M of precipitant, and (c) Obtaining T1 lipase crystals from step (b) by a hanging-drop methodology, wherein crystals of the lipase are formed between about 1 hour and 1 day. The method is used to generate crystals of the thermostable lipase from Geobacillus stearothermophilus strain T1, with crystallisation occurring at a range of temperatures between 16°C and 70°C.
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Additional Metadata
| Item Type: | Patent |
|---|---|
| Subject: | Biochemistry |
| Subject: | Microbiology |
| Subject: | Biotechnology |
| Application Number: | PC:T/MY2007/000043 |
| Filing Date: | 2007-06-20 |
| Divisions: | Universiti Putra Malaysia |
| Keywords: | T1 lipase crystallization; Geobacillus sp. strain T1; Thermostable lipase; Protein crystallization; Hanging drop method; Crystallization buffer; NaH2PO4; KH2PO4; MES buffer; NaCl |
| Sustainable Development Goals (SDGs): | SDG 9: Industry, Innovation and Infrastructure, SDG 3: Good Health and Well-being, SDG 17: Partnerships for the Goals |
| Depositing User: | Ms. Nur Aina Ahmad Mustafa |
| Date Deposited: | 13 Jul 2026 02:34 |
| Last Modified: | 13 Jul 2026 02:34 |
| URI: | http://psasir.upm.edu.my/id/eprint/127043 |
| Statistic Details: | View Download Statistic |
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