Citation
Raja Noor Zaliha Raja Abd Rahman
(2014)
Method for crystallizing geobacillus strain T1 polypeptide.
US:12/415,437.
Abstract
A method for crystallizing a Geobacillus strain T1 polypeptide under gravity or microgravity conditions is provided. A purified protein to be crystallized is provided, the purified protein being either thermostable T1 lipase or thermostable F16L lipase. A protein solution is prepared, comprising the purified protein, a buffer solution having a working pH of at least 8.5, and between 3-5% glycerol. A reservoir solution is prepared, comprising 0.1M MES, 0.1M NaH2PO4, 0.1M KH2PO4, and 1 M NaCl, and having a working pH of 6.5 or 6.6. The protein and reservoir solutions are combined to obtain a protein droplet, and protein crystals are obtained from the protein droplet by hanging-drop vapor diffusion.
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Additional Metadata
| Item Type: | Patent |
|---|---|
| Subject: | Biochemistry |
| Subject: | Biotechnology |
| Subject: | Crystallography |
| Application Number: | US:12/415,437 |
| Registration Number: | US:8920558 |
| Filing Date: | 2009-03-31 |
| Granted Date: | 2014-12-30 |
| Divisions: | Universiti Putra Malaysia |
| Keywords: | Geobacillus strain T1 polypeptide; Protein crystallization; Gravity; Microgravity; Thermostable lipase; Glycerol; Buffer solution; Vapor diffusion; Hanging-drop method; MES |
| Sustainable Development Goals (SDGs): | SDG 9: Industry, Innovation and Infrastructure, SDG 3: Good Health and Well-being, SDG 12: Responsible Consumption and Production |
| Depositing User: | Ms. Nur Aina Ahmad Mustafa |
| Date Deposited: | 06 Jul 2026 07:37 |
| Last Modified: | 06 Jul 2026 07:37 |
| URI: | http://psasir.upm.edu.my/id/eprint/126880 |
| Statistic Details: | View Download Statistic |
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