Citation
Raja Noor Zaliha Raja Abdul Rahman
(2014)
Process for producing lipase.
US:14/281,554.
Abstract
A process for producing lipase by microorganisms on the industrial scale, wherein Escherichia coli microorganism harbouring lipase, preferably thermostable lipase, is fermented under batch or fed batch conditions, in a Luria Bertani (LB) broth supplemented with citric acid, (NH 4 ) 2 SO 4 , Na 2 HPO 4 .12H 2 O, MgCl 2 , NH 4 Cl and glucose, using a trace metal solution containing hydrochloric acid, FeSO 4 .7H 2 O, ZnSO 4 .7H 2 0, CaCl 2 .H 2 0, MnSO 4 .5H 2 0, CuSO 4 .5H 2 O, AlCl.6H 2 O, and H 3 BO 3 , and a feeding nutrient solution containing glucose, yeast extract, MgSO 4 .H 2 O, KH 2 PO 4 , Citric Acid, (NH 4 ) 2 SO 4 , Na 2 HPO 4 .12H 2 O, MgCl 2 , and NH 4 Cl, at dissolved oxygen tension of 30-50%, at temperature of 30-40° C., while maintaining the pH between 6 and 8, with a glucose content of 15-25 g/L, with isopropylthiogalactoside.
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Additional Metadata
| Item Type: |
Patent
|
| Subject: |
Biotechnology |
| Subject: |
Biochemistry |
| Subject: |
Microbiology |
| Application Number: |
US:14/281,554 |
| Filing Date: |
2014-05-19 |
| Divisions: |
Universiti Putra Malaysia |
| Keywords: |
Lipase production; Escherichia coli; Fermentation; Batch culture; Fed-batch culture; Luria Bertani broth; Thermostable lipase; Trace elements; Nutrient solution; Industrial scale |
| Sustainable Development Goals (SDGs): |
SDG 9: Industry, Innovation and Infrastructure, SDG 3: Good Health and Well-being, SDG 12: Responsible Consumption and Production |
| Depositing User: |
Ms. Nur Aina Ahmad Mustafa
|
| Date Deposited: |
03 Jul 2026 02:00 |
| Last Modified: |
03 Jul 2026 02:00 |
| URI: |
http://psasir.upm.edu.my/id/eprint/126802 |
| Statistic Details: |
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