Hydroxyl radical-driven structural de-antigenicity of casein: A comparative ROS mechanism in argon plasma

Citation

Zhang, Le and Yang, Xinyu and Sun, Jiayu and Wen, Zimo and Tan, Chin Ping and Dang, Yali and Lai, Oi Ming and Liu, Aiming and Pan, Daodong and Du, Lihui (2026) Hydroxyl radical-driven structural de-antigenicity of casein: A comparative ROS mechanism in argon plasma. International Dairy Journal, 180. art. no. 106670. pp. 1-12. ISSN 0958-6946

Abstract

Casein (CN) is a major allergen in bovine milk, and reactive species generated by cold plasma have been shown to modulate its conformation and antigenicity. The effects of scavenging major reactive oxygen species (ROS) from cold argon plasma on CN modification and antigenicity were investigated in this study. Notably, scavenging hydroxyl radicals (·OH) partially reversed the plasma-induced reduction in IgE-binding capacity, with the value recovering from 24.37% to 80.44%. Structural analyses indicated that ·OH predominantly drives CN degradation and insoluble aggregation. Moreover, ·OH and singlet oxygen (1O2) induced irreversible oxidation and conformational rearrangement, while hydrogen peroxide (H2O2) caused mild, partially reversible oxidation. This study highlights irreversible oxidative damage by ROS and offers insights into antigenicity regulation during cold plasma treatment.

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Additional Metadata

Item Type:	Article
Subject:	Food Science
Subject:	Applied Microbiology and Biotechnology
Divisions:	Faculty of Food Science and Technology Institute of Bioscience
DOI Number:	https://doi.org/10.1016/j.idairyj.2026.106670
Publisher:	Elsevier Ltd
Keywords:	Antigenicity; Casein; Cold plasma; Epitopes; Reactive oxygen species
Sustainable Development Goals (SDGs):	SDG 2: Zero Hunger, SDG 3: Good Health and Well-being, SDG 9: Industry, Innovation and Infrastructure
Depositing User:	Ms. Siti Radziah Mohamed@mahmod
Date Deposited:	30 Jul 2026 02:11
Last Modified:	30 Jul 2026 02:11
Altmetrics:	https://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.idairyj.2026.106670
URI:	http://psasir.upm.edu.my/id/eprint/125945
Statistic Details:	View Download Statistic

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