Synergistic chemopreventive effects of curcumin, gingerol, and shogaol on HeLa cervical cancer cells

Objective: Bioactive compounds including curcumin, gingerols, and shogaols possess strong anti-inflammatory and anti-tumour properties. However, their combined efficacy has yet to be fully explored. This study aimed to investigate the chemopreventive potential of individual and combined treatments of 6-/10-gingerols (6-/10-G), 6-/10-shogaols (6-/10-S), and curcumin (Cur) in HeLa cervical cancer cells. Methods: HeLa cell viability was evaluated using the MTT assay following a 24-hour treatment with a wide range of concentrations and ratios of the bioactive compounds. The combination index was analysed using the Chou-Talalay method, and apoptotic cells were assessed via flow cytometry with Annexin V/FITC-PI staining. Results: We found that the optimal formulation of Cur in combination with all ginger bioactive compounds demonstrated synergistic inhibition of HeLa cancer cell growth at a 3:1 ratio of Cur to ginger bioactive compounds. Furthermore, treatment with the Cur:6-G (45μM: 40μM) and Cur:10-S (45μM: 20μM) combination formulations at a ratio of 3:1 significantly (p < 0.005) inhibited cell growth (72-77%) and induced apoptosis (60-87%). These findings highlight the potent anticancer properties of the combined formulations on HeLa cells, surpassing the efficacy of individual compounds which require relatively high concentrations. Conclusion: The combination of Cur and ginger bioactive compounds enhances anti-proliferative effects and induces apoptosis in HeLa cell lines.

Text 125817.pdf - Published Version Available under License Creative Commons Attribution Non-commercial. Download (1MB) Official URL or Download Paper: https://journal.waocp.org/article_91933.html

Actions (login required)

View Item