Antibacterial efficacy Oreochromis niloticus (Nile Tilapia) acid solubilized collagen towards Staphylococcus aureus and Pseudomonas aeruginosa skin infection in mice

Introduction: Oreochromis niloticus is one-third of the most aqua cultured fish around the world. Recent studies shows that Oreochromis niloticus extracted collagen from skin, scale, and bones shows promising quality for wound healing due to its biocompatibility. However, few studies are recorded regarding the antibacterial efficacies of the Oreochromis niloticus extracted collagen. The objective of the research is to evaluate the antibacterial efficacy of acid solubilized collagen extracted from Oreochromis niloticus scales toward Staphylococcus aureus and Pseudomonas aeruginosa skin infection in mice. Materials and Methods: 54 Balb-c mice weight around 25-30 g were divided equally under two bacteria groups, and further separated into three groups, which is positive, negative and tested group. The positive group were treated with acriflavine, tested group were treated with Oreochromis niloticus scales collagen, and the negative group were left untreated. The treatment were given everyday and the duration of treatment were performed for nine days. In every three days interval of treatment, swab sample on the skin infection of mice were taken to perform the Colony Formation Unit (CFU) counting. The data recorded were analysed using analysis of variance (ANOVA) test and student T-test. Results: There are no significance differences between the antibacterial efficacies between the treatments for all mice groups. However, acriflavine shows the highest bacterial efficacy towards Staphylococcus aureus and Pseudomonas aeruginosa skin infection on mice, followed by acid solubilized collagen treatment. Conclusion: Oreochromis niloticus scale extraction acid solubilized collagen shows low antibacterial efficacy towards Staphylococcus aureus and Pseudomonas aeruginosa skin infection.

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