The impact of adding egg yolk in various concentrations into the Tyrode extender on Muscovy duck (Cairina moschata) sperm quality

This study investigated the impact of varying egg yolk concentrations in Tyrode extender on the quality of Muscovy duck semen after preservation. A sample of semen was collected from 1.5-year-old Muscovy ducks. The sample was evaluated for sperm motility, plasma membrane integrity, acrosome integrity, and sperm morphology before being separated into three equal parts and diluted in Tyrode extender containing increasing levels of egg yolk: T1 (5%), T2 (10%), and T3 (15%). After 21 hours of preservation at 4-5ºC, each sample was evaluated for the same parameters as fresh semen. The study found a significant decrease in sperm motility, membrane integrity, and intact acrosome cap following preservation in T1, T2, and T3 compared to fresh ejaculate (53.34%; 63.5%; 60.04% vs 84.16%), (48.7%; 57.8%; 55.3% vs. 85.6%), and (50.6%; 57.6%; 56.1% vs 85.4%), respectively (P<0.05). In contrast, abnormal sperm morphology increased considerably in T1, T2, and T3 compared to fresh semen (6.69%, 6.89%, 7.89% vs 2.2%) (P<0.05). Furthermore, the addition of 10% egg yolk to the Tyrode extender resulted in the best percentages of sperm motility, sperm membrane integrity, and intact acrosome cap following preservation. Surprisingly, the percentage of sperm with abnormal morphology did not differ significantly (P>0.05) between T1, T2, and T3 after the preservation period. It can be concluded that adding 10% egg yolk to the extender substantially improved sperm motility, membrane integrity, and intact acrosome cap in Muscovy duck sperm after 21 hours of preservation.

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