Secondary metabolites of endophytic Pseudomonas aeruginosa XGDE 7 from mangrove Xylocarpus granatum J. Koenig: antibacterial, antibiofilm, and antioxidant activities

Xylocarpus granatum is a true mangrove species that belongs to Meliaceae family. X.granatum serves limonoids compounds commonly utilized by coastal communities as traditional medicine. This study is designed to investigate antioxidant, antibacterial, and antibiofilm activities of bioactive compounds produced by associated endophytic bacteria isolated from X.granatum leaves. The endophytic bacteria isolate XGDE 7 was obtained from the collection of microbiology laboratory, Department of Biology, University of Bengkulu. Based on 16sRNA analysis, this isolate is closely related to P.aeruginosa strain BSP12 with similarity of 99.82 %. The antibacterial activity using pellet and supernatant exhibited that the widest inhibition zone diameters were found in inhibiting Bacillus subtilis (7.93 ± 0.42 mm; 3.70 ± 0.16 mm). Furthermore, the largest clear zone using crude extract was shown against Escherhicia.coli (12.29 ± 0.50 mm). The lowest MIC of XGDE 7 was found on Eenterococcus faecalis (15.62 µg/ml) and the highest MIC was on Stapylococcus aures (62.50 µg/ml). As for MBC, XGDE 7 isolate showed the lowest activity on E.faecalis (31.25 µg/ml), while the highest was observed on MBC S. aureus (125 µg/ml). Antibiofilm activity of XGDE 7 extract S.aureus, B.subtilis, E.coli and E.faecalis effectively inhibited at a concentration range of 2 x MIC with inhibition range of 80.10±0.5 to 74.79 ± 0.1%. Meanwhile, the antioxidant activity using DPPH method exhibited lC50 of 49.25 µg/ml. GC-MS analysis revealed various bioactive compounds; Cyclo (L-prolyl-L-valine), Hexadecanoic acid, methyl ester, Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl), 9-octadecenoic acid (z)-,methyl ester, 10-Octadecenoic acid,methyl ester, 2,5-Piperazinedione, 3,6-bis (2-methylpropyl), and Pyrrolo [1,2-a]pyrazine-1,4-dione, hexahydro-3(phenylmethyl).

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