UPM Institutional Repository

Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat


Chandrasiri, Alawattage Don Nimal (1997) Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat. PhD thesis, Universiti Putra Malaysia.


A study was conducted to determine if goat ovaries obtained from a slaughterhouse could be used for in vitro fertilisation (lVF). Three experiments were conducted to ascertain if a substance named S-ACT-l, extracted from a marine algae, (Gelidiella acerosa), with a chemical structure similar to heparin could be used for sperm capacitation. The acrosome reaction was tested by a dual staining technique (Experiment 1 ) and the zona free hamster penetration test (Experiment 2). In vitro maturation o f goat oocytes and fertilisation were assessed by the number of zygotes cleaving beyond the 2-cell stage (Experiment 3). Freshly ejaculated semen from two fertile bucks were used for sperm capacitation using two defined media (DM-H and DM-Ca). Heparin and S-ACT-l were tested at concentrations of 10, 20, 50 and 100 ug/ml. After incubating for 15 min. at 39° C with 5 % CO2 in air under maximum humidity, samples were stained with trypan blue and Giemsa stain (dual stain). The results indicated that the sperm treated with heparin and S-ACT - 1 showed similar staining characteristics.

Download File

[img] PDF

Download (760kB)

Additional Metadata

Item Type: Thesis (PhD)
Subject: Goats - Fertility
Subject: Fertilization in vitro
Call Number: FPV 1997 6
Chairman Supervisor: Professor Tengku Azmi bin Tengku Ibrahim, PhD
Divisions: Faculty of Veterinary Medicine
Depositing User: Mohd Nezeri Mohamad
Date Deposited: 13 Jul 2011 01:22
Last Modified: 12 Sep 2011 01:34
URI: http://psasir.upm.edu.my/id/eprint/12327
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item