Simple Search:

Isolation and characterization of an acrylamide-degrading Antarctic bacterium


Citation

Abd. Shukor, Mohd Yunus and Gusmanizar, Neni and Ramli, Johari and Shamaan, Nor Aripin and MacCormack, W. P. and Syed, Mohd Arif (2009) Isolation and characterization of an acrylamide-degrading Antarctic bacterium. Journal of Environmental Biology, 30 (1). pp. 107-112. ISSN 0254-8704

Abstract / Synopsis

The presence of acrylamide in the environment poses a threat due to its well known neurotoxic, carcinogenic and teratogenic properties. Human activities in various geographical areas are the main anthropogenic source of acrylamide pollution. In this work, an acrylamide-degrading bacterium was isolated from Antarctic soil. The physiological characteristics and optimum growth conditions of the acrylamide-degrading bacteria were investigated. The isolate was tentatively identified as Pseudomonas sp. strain DRYJ7 based on carbon utilization profiles using Biolog GN plates and partial 16S rDNA molecular phylogeny. The results showed that the best carbon sources for growth was glucose and sucrose with no significant difference in terms of cellular growth between the two carbon sources (p>0.05). This was followed by fructose and maltose with fructose giving significantly higher cellular growth compared to maltose (p<0.05). Lactose and citric acid did not support growth. The optimum acrylamide concentration as a nitrogen source for cellular growth was at 500 mgl-1. At this concentration, bacterial growth showed a 2-day lag phase before degradation took place concomitant with an increase in cellular growth. The isolate exhibited optimum growth in between pH 7.5 and 8.5. The effect of incubation temperature on the growth of this isolate showed an optimum growth at 15°C. The characteristics of this isolate suggest that it would be useful in the bioremediation of acrylamide


Download File

[img]
Preview
PDF
Journal_of_Environmental_Biology.pdf

Download (202kB)

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Publisher: Triveni Enterprises, Lucknow
Keywords: Acrylamide, Biodegradation, Pseudomonas sp., Antarctica
Depositing User: Najwani Amir Sariffudin
Date Deposited: 21 Apr 2011 09:05
Last Modified: 27 May 2013 07:50
URI: http://psasir.upm.edu.my/id/eprint/11992
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item