Simple Search:

Expression Profiles of Wnt Genes during Neural Differentiation of Mouse Embryonic Stem Cells


Citation

Nordin, Norshariza and Meng, Li and Mason, John O. (2008) Expression Profiles of Wnt Genes during Neural Differentiation of Mouse Embryonic Stem Cells. Cloning and Stem Cells, 10 (1). pp. 37-48. ISSN 1178-6957

Abstract / Synopsis

The Wnt family of secreted signaling proteins regulates many aspects of animal development and the behavior of several types of stem cells, including embryonic stem (ES) cells. Activation of canonical Wnt signaling has been shown to either inhibit or promote the differentiation of ES cells into neurons, depending on the stage of differentiation. Here, we describe the expression of all 19 mouse Wnt genes during this process. Using the well-established retinoic acid induction protocol we found that all Wnt genes except Wnt8b are expressed as ES cells differentiate into neurons, many of them in dynamic patterns. The expression pattern of 12 Wnt genes was analyzed quantitatively at 2-day intervals throughout neural differentiation, showing that multiple Wnt genes are expressed at each stage. A large proportion of these, including both canonical and noncanonical Wnts, are expressed at highest levels during later stages of differentiation. The complexity of the patterns observed indicates that disentangling specific roles for individual Wnt genes in the differentiation process will be a significant challenge.


Download File

[img]
Preview
PDF (Abstract)
Expression Profiles of Wnt Genes during Neural Differentiation of Mouse Embryonic Stem Cells.pdf

Download (83kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1089/clo.2007.0060
Depositing User: Muizzudin Kaspol
Date Deposited: 19 Apr 2011 07:58
Last Modified: 22 Dec 2015 04:35
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1089/clo.2007.0060
URI: http://psasir.upm.edu.my/id/eprint/11834
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item