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Improvement Of Molecular Methods For Detection Of Pathogenic Escherichia Coli.


Citation

Abdullah Al-Haj, Nagi Ahmed (2007) Improvement Of Molecular Methods For Detection Of Pathogenic Escherichia Coli. PhD thesis, Universiti Putra Malaysia.

Abstract / Synopsis

Diarrhea is one of the leading causes of illnesses and death among children in developing countries, where an estimated 1.3 billion episodes and 4 to 10 million deaths occur each year in children below 5 years of age. The common pathogens of diarrhea are diarrheagenic Escherichia coli (DEC), Group A rotavirus, Shigella spp, Salmonella spp, Campylobacter, and Vibrio cholerae. Microbiological insights including phenotypic and genotypic characterisation are valuable approaches with application in management and prevention of diarrheal outbreaks by E. coli. In the present study, the Random Amplified polymorphic DNA (RAPD) fingerprinting technique allowed genetic diversity assessment of 25 E. coli isolates. Six out of 20 arbitrary primers namely, OPAE 4, 9, 10, 11, 12 and 18 produced DNA fingerprinting patterns providing the discriminatory power and the display of the potential epidemiological and diagnostic markers. A highly significant finding from the DNA fingerprinting is the display of a predominant band at a size of 308 bp when arbitrary


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Additional Metadata

Item Type: Thesis (PhD)
Subject: Enteropathogenic Escherichia coli
Call Number: FPSK(P) 2007 3
Chairman Supervisor: Associate Professor Mariana Nor Shamsudin, PhD
Divisions: Faculty of Medicine and Health Science
Depositing User: Mohd Nezeri Mohamad
Date Deposited: 18 Jul 2011 09:52
Last Modified: 17 Sep 2014 15:24
URI: http://psasir.upm.edu.my/id/eprint/11279
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