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Establishment Of An In Vitro Cell Culture System For Human Hepatitis B Virus


Wang, Suk Mei (2001) Establishment Of An In Vitro Cell Culture System For Human Hepatitis B Virus. Masters thesis, Universiti Putra Malaysia.


The major obstacle in the study of HBV has been the inability to infect either animal model system in vivo or continuous cell lines in vitro due to strict virushost range and preferential attachment of the HBV envelope proteins onto the plasma membrane of human hepatocytes. The direct involvement of human annexin-V, a calcium dependent phospholipid - binding protein in the initial step of HBV infection has been reported. Over the last decade, various culture conditions with exogenous soluble factors have also been exploited to enhance HBV infection in vitro. The aim of this study was to establish an in vitro cell culture system that would continuously produce sufficient HBV by episomal replication. To facilitate the penetration and internalization of HBV, the expression of annexin V (A V) was

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Additional Metadata

Item Type: Thesis (Masters)
Call Number: FPSK(M) 2001 13
Chairman Supervisor: Associate Professor Dr. Seow Heng Fong
Divisions: Faculty of Medicine and Health Science
Depositing User: Mohd Nezeri Mohamad
Date Deposited: 18 Jul 2011 03:36
Last Modified: 08 Aug 2011 05:06
URI: http://psasir.upm.edu.my/id/eprint/11228
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