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Analysis of genetic variation of inducible nitric oxide synthase and natural resistance-associated macrophage protein 1 loci in Malaysian native chickens


Citation

Tohidi, Reza and Idris, Ismail and Panandam, Jothi Malar and Bejo, Mohd Hair (2011) Analysis of genetic variation of inducible nitric oxide synthase and natural resistance-associated macrophage protein 1 loci in Malaysian native chickens. African Journal of Biotechnology, 10 (8). art. no. 89EB5BE25532. pp. 1285-1289. ISSN 1684–5315

Abstract / Synopsis

The genetic diversity of 100 Malaysian native chickens was investigated using polymerase chain reaction-restriction fragment polymorphism (PCR-RFLP) for two candidate genes: inducible nitric oxide synthase (INOS) and natural resistance-associated macrophage protein 1 (NRAMP1). The two genes were selected because of their important role in chicken's immune system. INOS and NRAMP1 PCR products were digested by AluI and SacI restriction enzymes, respectively. The restriction digests produced fragment sizes of 322 and 173 bp for INOS and 722 and 79 bp for NRAMP1 as one allele and an undigested PCR product as the other allele. Both loci were polymorph, however only INOS gene showed Hardy-Weinberg equilibrium. Average heterozygosity and the Shannon information index (I) was 0.43 and 0.62 for INOS and 0.48 and 0.68 for NRAMP1 genes, respectively. The observed polymorphism in this study shows the ability of these candidate genes in marker assisted selection and introgression programs to increase resistance to diseases in both Malaysian native and commercial chickens.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Veterinary Medicine
Institute of Tropical Agriculture
DOI Number: https://doi.org/10.5897/AJB10.829
Publisher: Academic Journals
Keywords: Malaysian native chickens; Polymorphism; Inducible nitric oxide synthase (INOS); Natural resistance-associated macrophage protein 1 (NRAMP1)
Depositing User: Azizan Arshad
Date Deposited: 25 Mar 2011 07:21
Last Modified: 13 Nov 2017 04:14
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5897/AJB10.829
URI: http://psasir.upm.edu.my/id/eprint/11130
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