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Devising a colorimetric aptasensor for detection of basal stem rot-associated RNA marker during early Ganoderma boninense infection in oil palm


Citation

Bahari, Mohammad Nazri Abdul and Abdullah, Siti Nor Akmar and Sakeh, Nurshafika Mohd and Ahmad, Khairulmazmi and Shaharuddin, Noor Azmi and Abu Seman, Idris and Osman, Rosiah (2023) Devising a colorimetric aptasensor for detection of basal stem rot-associated RNA marker during early Ganoderma boninense infection in oil palm. Physiological and Molecular Plant Pathology, 129. art. no. 102209. pp. 1-13. ISSN 0885-5765

Abstract

Basal stem rot (BSR) is a detrimental disease in oil palm (Elaeis guineensis) due to the host's lack of symptoms during the early phase of infection. Early detection of BSR at non-symptomatic phase is feasible via molecular-based approaches and has become the major interest among researchers. Our previous study had identified a differentially expressed gene encoding auxin efflux carrier component 5-like (EgPIN5) at 11 day-post-inculation of Ganoderma boninense (symptomless early stage of infection) on oil palm seedlings. We further evaluated the gene expression on samples collected from an oil palm plantation at different BSR stages (symptomless, mild and severe). Our findings revealed that EgPIN5 was expressed in symptomless group but was not expressed in either mild or severe BSR groups. Next, we developed a sandwich-type aptamer-based lateral flow assay (LFA) against EgPIN5 RNA by adapting the versatile DNA-functionalized gold nanoparticles for colorimetric detection. The LFA strip demonstrated visible colorimetric signal of EgPIN5 RNA at 12.5 ng/μL with limit of detection at 2.45 ng/μL using a scanning device. Based on our consistent results, we presented EgPIN5 as a diagnostic biomarker for symptomless BSR infection in oil palm. Utilization of LFA assay strip for practical EgPIN5 RNA detection is hoped to encourage establishment of a fast, cheap, and sensitive point-of-care detection system in plant biology. © 2023 Elsevier Ltd


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Biotechnology and Biomolecular Sciences
Institute of Advanced Technology
Institute of Plantation Studies
DOI Number: https://doi.org/10.1016/j.pmpp.2023.102209
Publisher: Elsevier
Keywords: Aptame; rAuxin efflux carrier component 5; Basal stem rot; Lateral flow assay; Oil palm
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 29 Mar 2024 03:49
Last Modified: 30 May 2024 03:45
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.pmpp.2023.102209
URI: http://psasir.upm.edu.my/id/eprint/105720
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