Citation
Abstract
Aim and objective: Due to the a lot of unexplored proteins in HIV-1, this research aimed to explore the functional roles of a hypothetical protein (AAB33144.1) that might play a key role in HIV-1 pathogenicity. Methods: The homologous protein was identified along with building and validating the 3D structure by searching several bioinformatics tools. Results: Retroviral aspartyl protease and retropepsin like functional domains and motifs, folding pattern (cupredoxins), and subcellular localization in cytoplasmic membrane were determined as biological activity. Besides, the functional annotation revealed that the chosen hypothetical protein possessed protease-like activity. To validate our generated protein 3D structure, molecular docking was performed with five compounds where nelfinavir showed (−8.2 kcal/mol) best binding affinity against HXB2 viral protease (PDB ID: 7SJX) and main protease (PDB ID: 4EYR) protein. Conclusions: This study suggests that the annotated hypothetical protein related to protease action, which may be useful in viral genetics and drug discovery. © 2023
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Additional Metadata
Item Type: | Article |
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Divisions: | Faculty of Biotechnology and Biomolecular Sciences |
DOI Number: | https://doi.org/10.1016/j.heliyon.2023.e23183 |
Publisher: | Elsevier |
Keywords: | AAB33144.1; Cupridoxins; Cytoplasmic membrane; HXB2 strain; Hypothetical protein; Protease |
Depositing User: | Ms. Nuraida Ibrahim |
Date Deposited: | 15 Feb 2024 04:21 |
Last Modified: | 15 Feb 2024 04:21 |
Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.heliyon.2023.e23183 |
URI: | http://psasir.upm.edu.my/id/eprint/105704 |
Statistic Details: | View Download Statistic |
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