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Efficacy of single and multi-strain probiotics on in vitro strain compatibility, pathogen inhibition, biofilm formation capability, and stress tolerance


Citation

Puvanasundram, Puvaneswari and Chong, Chou Min and Sabri, Suriana and Mohd Yusoff, Md Sabri and Lim, Keng Chin and Karim, Murni (2022) Efficacy of single and multi-strain probiotics on in vitro strain compatibility, pathogen inhibition, biofilm formation capability, and stress tolerance. Biology, 11 (11). art. no. 1644. pp. 1-16. ISSN 2079-7737

Abstract

Compatibility of each strain in a multi-strain probiotic (MSP), along with its properties, becomes a strong base for its formulation. In this study, single-strain probiotics (SSPs) and multi-strain probiotics (MSPs) were evaluated in vitro for strain compatibility, microbial antagonism, biofilm formation capacity, and stress tolerance. Bacillus amyloliquefaciens L11, Enterococcus hirae LAB3, and Lysinibacillus fusiformis SPS11 were chosen as MSP1 candidates because they showed much stronger antagonism to Aeromonas hydrophila and Streptococcus agalactiae than a single probiotic. MSP 2 candidates were Lysinibacillus fusiformis strains SPS11, A1, and Lysinibacillus sphaericus strain NAS32 because the inhibition zone produced by MSP 2 against Vibrio harveyi and Vibrio parahaemolyticus was much higher than that produced by its constituent SSPs. MSP1 in the co-culture assay reduced (p < 0.05) A. hydrophila count from 9.89 ± 0.1 CFU mL−1 to 2.14 ± 0.2 CFU mL−1. The biofilm formation of both MSPs were significantly higher (p < 0.05) than its constituent SSPs and the pathogens. The SSPs in both MSPs generally showed resistance to high temperatures (80, 90, and 100 °C) and a wide range of pH (2 to 9). This in vitro assessment study demonstrates that MSP1 and 2 have the potential to be further explored as multi-strain probiotics on selected aquatic species.


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Official URL or Download Paper: https://www.mdpi.com/2079-7737/11/11/1644

Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Biotechnology and Biomolecular Sciences
Faculty of Veterinary Medicine
Institute of Bioscience
International Institute of Aquaculture and Aquatic Science
DOI Number: https://doi.org/10.3390/biology11111644
Publisher: Multidisciplinary Digital Publishing Institute
Keywords: Multi-strain probiotics (MSP); Single-strain probiotics (SSPs); Antagonism; Strain compatibility; Properties; Biofilm; Stress tolerance
Depositing User: Ms. Che Wa Zakaria
Date Deposited: 22 Sep 2023 23:37
Last Modified: 22 Sep 2023 23:37
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/biology11111644
URI: http://psasir.upm.edu.my/id/eprint/101213
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