Optimization of Biohydrogen Production from Palm Oil Mill Effluent by Natural Microflora

Rasdi, Zatilfarihiah (2009) Optimization of Biohydrogen Production from Palm Oil Mill Effluent by Natural Microflora. Masters thesis, Universiti Putra Malaysia.

[img] PDF
431Kb

Abstract

Biohydrogen is a promising clean fuel as it is ultimately derived from renewable energy sources. It is environmental friendly since it burns to water, gives high energy yield, and can be produced by less energy-intensive processes. Anaerobic treatment of palm oil mill effluent (POME) was chosen to produce biohydrogen as POME is a commercially known waste that is such a burden to the industry and the environment. In this study, POME sludge was used as an inoculum to produce biohydrogen from POME. Heat-treated POME sludge acclimatised with POME incubated at 37°C for 24 h was used as a seed culture. Preliminary screening on the effects of inocula size, heat treatment, substrate concentration and pH of incubation by using a factorial design (FD) were conducted under mesophilic condition (37°C) using a serum vial (160 mL). The experimental results from two-level FD showed that pH and chemical oxygen demand (COD) of POME as substrate concentration significantly affected biohydrogen production. Optimizations of the specific hydrogen production (Ps) and the hydrogen production rate (Rm) were carried out by using a central composite design (CCD). The maximum Ps of 270 mL H2/g carbohydrate and Rm of 98 mL H2/h were obtained under optimum conditions of pH 5.86 and substrate concentration of 80 g/L. The optimized conditions obtained were subjected to a confirmation run and it showed a reproducible data with Ps of 282 mL H2/g carbohydrate and Rm of 137 mL H2/h. For the second part of experiment, 2-L of bioreactor was employed for the production of biohydrogen with and without pH control. The optimum conditions obtained in the serum vial were applied in the bioreactor. The results obtained for uncontrolled pH experiment generated 1.3 L biogas/L medium. Throughout the fermentation, no methane-gas was detected. The biohydrogen yield (Ps) was approximately 1 L H2/L medium, with hydrogen production rate (Rm) at 112 mL H2/h. For the controlled pH experiment, pH was controlled manually every 3 h at 5.86. The biogas generated from the fermentation was 2.5 L/L medium, which is almost 2-fold of biogas production from uncontrolled pH experiment. The Ps and Rm generated were 1.3 L H2/L medium and 144 mL H2/h, respectively.

Item Type:Thesis (Masters)
Subject:Hydrogen - Biotechnology
Subject:Palm - oil mill effluent
Chairman Supervisor:Dr. Nor’Aini Abdul Rahman
Call Number:FBSB 2009 32
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
ID Code:9825
Deposited By: Laila Azwa Ramli
Deposited On:23 Feb 2011 05:43
Last Modified:27 May 2013 07:43

Repository Staff Only: item control page

Document Download Statistics

This item has been downloaded for since 23 Feb 2011 05:43.

View statistics for "Optimization of Biohydrogen Production from Palm Oil Mill Effluent by Natural Microflora"


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.