Molecular Genetic Characterization of Different Accessions of Centella Asiatica

Wong, Sook Mun (2003) Molecular Genetic Characterization of Different Accessions of Centella Asiatica. Masters thesis, Universiti Putra Malaysia.

[img] PDF
985Kb

Abstract

Centella asiatica or locally known as "pegaga" belongs to the Apiceae family. This medicinal plant is one of the most important medicinal herbs, and widely used in health foods, pharmaceutical and cosmetic industries. Twelve accessions of C. asiatica planted in MARDI, originated from different locations in Peninsular Malaysia, were used for this study. Phenotypic differences among these accessions are not very distinct, thus this study is undeitaken to determine whether there are any genetic differences. DNA markers, unaff ected by environmental or physiological factors, have potential utility in the characterization of plant species. High discriminating power of this class of markers demonstrated uniformity and stability within genetically complex cultivars. Good quality DNA was extracted from leaf samples using conventional hexadecyltrimethylammonium bromide (CTAB) method. Two PeR-based DNA markers system. namely Amplified Fragment Length Polymorphisms (AFLPs) and Long Primer Randomly Amplified Polymorphic DNA (LP-RAPDs), were employed. This study has successfully analyzed the genetic relationships among the accessions of C. asiatica. Two phylogenetic trees bad been constructed from the unweighted pair group method with arithmatical average (UPGMA) pairwise analyses. Genetic distances was calculated based on. the Dice similarity index. From both analyses, the CA01 and CA02 as wen as CA05 and CA06 were closer (D=0.119) within the same cluster indicating that they are closely related. Based on the genetic distances, CA10 represented as highest distant group in the LP-RAPDs analyses whereas CA03 represented as highest distant group in the AFLPs analyses. Furthermore, diagnostic band with highest molecular weight (3000 bp) was found in CA10 by using long primer PEH A3. The amplification of CA03 genotypes with AFLP primer pair ACG/CTA has shown a unique DNA profile. In addition, CA03 is easily distinguish from other accessions morphologically due to its wavy shape of the plant leaf.

Item Type:Thesis (Masters)
Subject:Molecular genetics - Malaysia
Subject:Plants - Classification - Molecular aspects - Malaysia
Chairman Supervisor:Associate Professor Siti Khalijah Daud, PhD
Call Number:FSAS 2003 12
Faculty or Institute:Faculty of Environmental Studies
ID Code:9542
Deposited By: Muizzudin Kaspol
Deposited On:14 Feb 2011 08:39
Last Modified:21 May 2012 03:32

Repository Staff Only: Edit item detail

Document Download Statistics

This item has been downloaded for since 14 Feb 2011 08:39.

View statistics for "Molecular Genetic Characterization of Different Accessions of Centella Asiatica"


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.