Nucleocapsid Protein of Newcastle Disease Virus as an Antigenic Carrier

Rabu, Amir (2002) Nucleocapsid Protein of Newcastle Disease Virus as an Antigenic Carrier. Masters thesis, Universiti Pertanian Malaysia.

[img] PDF
1915Kb

Abstract

Newcastle disease virus (NDV) is an economically important avian virus that causes loss to the poultry industry. It has a wide host range infecting 27 of the 50 orders of birds. Generally, the virus consists of six structural proteins: nucleocapsid (NP), phosphoprotein (P), matrix (M), fusion (F), haemagglutinin-neuramidase (HN) and large (L). The NP protein resembles the classical herringbone morphology when observed under electron microscope. However, the morphology changed into individual ring-like particles when the myc epitope and six histidine residues were fused to the C-terminal end of the protein. Further investigation showed that the C-terminus of this protein derivative is exposed on the surface of the ring-like particles. In this project, several chimeric proteins have been constructed in which the antigenic regions of the HN or F protein of NDV strain AF2240, myc epitope and six histidine residues were linked to the C-tenninus of the NP protein. The chimeric proteins were expressed efficiently in Escherichia coli as detected by Western blot analysis. Electron microscopic analysis on these proteins revealed that they assembled into ring-like particles. These chimeric NP proteins exhibited antigenicity of the myc epitope suggesting that the foreign sequences were exposed on the surface of the particles. Chickens vaccinated with the chimeric particles exhibited an immune response against NDY. However, no protection was observed when the vaccinated chickens were challenged by the virus.

Item Type:Thesis (Masters)
Subject:Newcastle disease virus
Subject:Cytoskeletal proteins
Chairman Supervisor:Khatijah Mohd Yusoff, PhD
Call Number:FSAS 2002 31
Faculty or Institute:Faculty of Science and Environmental Studies
ID Code:9491
Deposited By: Muizzudin Kaspol
Deposited On:10 Feb 2011 05:04
Last Modified:01 Oct 2013 01:28

Repository Staff Only: Edit item detail

Document Download Statistics

This item has been downloaded for since 10 Feb 2011 05:04.

View statistics for "Nucleocapsid Protein of Newcastle Disease Virus as an Antigenic Carrier"


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.