Typhonium flagelliforme inhibits the proliferation of murine leukemia WEHI-3 cells in vitro and induces apoptosis in vivo

Mohan, Syam and Abdul, Ahmad Bustamam and Abdel Wahab, Siddig Ibrahim and Al-Zubairi, Adel Sharaf and Sukari, Mohamed Aspollah and Abdullah, Rasedee and Elhassan Taha, Manal Mohamed and Ng, Kuan Beng and Mohd Isa, Nurbaity (2010) Typhonium flagelliforme inhibits the proliferation of murine leukemia WEHI-3 cells in vitro and induces apoptosis in vivo. Leukemia Research, 34 (11). pp. 1483-1492.

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1016/j.leukres.2010.04.023

Abstract

Typhonium flagelliforme (TF) is a tropical plant, traditionally used by the ethnic population of Malaysia for the cure of various cancers. This plant had shown to induce antiproliferative effect as well as apoptosis in cancer cells. However, there is no available information to address that TF affects murine leukemia cells in vitro and in vivo. Here, we investigated in vitro and in vivo effects of TF on murine leukemia WEHI-3 cells. It was found that the growth of leukemia cells in vitro was inhibited by the various extracts of TF. Among these fractions, the dichloromethane (DCM) tuber extracts of TF showed the lowest IC50 (24.0±5.2g/ml) and had demonstrated apoptogenic effect when observed under fluorescent microscope.Weinvestigated the in vivo effects of DCM tuber extracts of TF on murine leukemia cells, and the results showed that the counts of immature granulocytes and monocytes were significantly decreased in peripheral blood of BALB/c leukemia mice after the oral administration of DCM tuber extracts of TF for 28 days with three doses (200, 400 and 800 mg/kg). These results were confirmed by observing the spleen histopathology and morphology of enlarged spleen and liver in leukemia micewhencompared with the control. Furthermore, the cell death mechanism in the spleen tissue of treated mice was found via apoptosis.

Item Type:Article
Keyword:Apoptosis, BALB/c mice, Cytotoxicity, Typhonium flagelliforme, WEHI-3 leukemia cells
Faculty or Institute:Faculty of Veterinary Medicine
Publisher:2010 Elsevier Ltd. All rights reserved
DOI Number:10.1016/j.leukres.2010.04.023
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.leukres.2010.04.023
ID Code:9453
Deposited By: Azizan Arshad
Deposited On:08 Feb 2011 02:25
Last Modified:08 Feb 2011 02:30

Repository Staff Only: Edit item detail


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.