Development of PCR-BASED DNA Markers to Identify Aand Characterise Malaysian River Catfish, Mystus Nemurus (C&V) : RAPD and AFLP

Chong, Lee Kim (1998) Development of PCR-BASED DNA Markers to Identify Aand Characterise Malaysian River Catfish, Mystus Nemurus (C&V) : RAPD and AFLP. Masters thesis, Universiti Pertanian Malaysia.

[img] PDF
1089Kb

Abstract

The main objectives of this study are to evaluate the usefulness of a variety of recently developed DNA markers to identify and characterise different populations of Malaysian river catfish, Mystus numerus. In the initial methodology development, 111 primers comprising of 40 single RAPD primers, 64 pairs of AFLP primers and 7 pairs of African catfish microsatellite primers were screened against M. nemurus samples. RAPD and AFLP primers gave positive results. Both techniques were also successfully used in examining the genetic diversity present in five populations of M. nemurus originating from Kedah. Perak, Johor, Sarawak and one UPM culture population. However, none of the micro satellite primers developed for the African catfish was suitable for typing our local M. nemurus. Therefore, this technique was not used for the population studies and emphasis was only given to RAPD and AFLP markers in this thesis. Nine RAPD primers and four AFLP primers detected a total of 42 and 158 polymorphic markers respectively. The modes of inheritance of the bands produced by four out of the nine RAPD primers and two out of the four AFLP primers were studied using family samples. The results showed that 9 of the RAPD markers and 24 of the AFLP markers used in population studies segregated as stable Mendelian loci while 2 RAPD markers and 13 AFLP markers showed unusual segregation. The rest of the markers could not be examined because no segregation was found in the families.

Item Type:Thesis (Masters)
Subject:DNA polymerases
Subject:Bagre - Malaysia
Chairman Supervisor:Professor Tan Soon Guan, PhD
Call Number:FSAS 1998 17
Faculty or Institute:Faculty of Science and Environmental Studies
ID Code:9423
Deposited By: Laila Azwa Ramli
Deposited On:01 Feb 2011 08:39
Last Modified:25 Sep 2013 02:24

Repository Staff Only: item control page


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.