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Process improvement in the production of oleyl oleate, a liquid wax ester in stirred tank reactor

Basri, Mahiran and Mat Radzi, Salina and Salleh, Abu Bakar and Ariff, Arbakariya and Mohamad, Rosfarizan and Abdul Rahman, Mohd Basyaruddin and Raja Abdul Rahman, Raja Noor Zaliha (2008) Process improvement in the production of oleyl oleate, a liquid wax ester in stirred tank reactor. In: 13th International Biotechnology Symposium & Exhibition, 12-17 Oct. 2008, Dalian, China.

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Lipase-catalyzed production of oleyl oleate, a liquid wax ester by esterification of oleic acid with oleyl alcohol was performed in 2 L stirred tank reactor (STR). Process improvement study in reactor operation was carried out in batch mode STR with single impeller mounted on the centrally located shaft. Rushton turbine impeller showed better performance in degree of mixing, as compared to AL Hydrofoil impeller, whereby a high Reynolds number of >104 was achieved at 400 rpm, which exhibit a turbulent flow pattern [1]. Homogenous enzyme particles suspension was obtained at lower impeller speed (≤200 rpm) by using AL Hydrofoil impeller. However, there was significant effect to the mixing improvement on the enzyme particles distribution by using 2 impellers system with spacing of 30 mm even at lower agitation speed (≤200 rpm). High stability of Novozym 435 was observed even in the presence of shear forces on the enzyme particles due to the mechanical agitation speed. Wax ester reaction mixture in STR follows Newtons’ law due to the linear relation between the shear stress (τ) and shear rate (dυ/dy) [2]. The process was successfully carried out to the highest amount of wax ester that can be produced in 2 L STR whereby, the production and productivity of oleyl oleate were improved from 295.39 g/L/h to 705.76 g/L/h and 310.16 g/h to 952.78 g/h, respectively.

Item Type:Conference or Workshop Item (Paper)
Subject:Oleic acid
Faculty or Institute:Faculty of Science
DOI Number:10.1016/j.jbiotec.2008.07.643
ID Code:8819
Deposited By: Samsida Samsudin
Deposited On:21 Dec 2010 01:34
Last Modified:05 Jan 2015 04:33

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