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Spectroscopic study of lipase from Candida rugosa immobilized onto calcined layered double hydroxides.

Abdul Rahman, Mohd Basyaruddin and Md. Yunus, Noor Mona and Basri, Mahiran and Hussein, Mohd Zobir and Salleh, Abu Bakar and Raja Abdul Rahman, Raja Noor Zaliha (2007) Spectroscopic study of lipase from Candida rugosa immobilized onto calcined layered double hydroxides. In: Enzyme Engineering XIX, 23-28 Sep. 2007, Harrison Hot Springs, British Columbia, Canada.

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Abstract

Lipase from Candida rugosa (CRL) was adsorbed onto three calcined layered double hydroxide (CLDH); Mg/Al-NO3 (CMAN), Ni/Al-NO3- (CNAN) and Zn/Al-NO3- (CZAN). Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy (SEM-EDX) and protein staining-light microscopy analyses were applied to provide direct spectroscopic and microscopic evidence for the adsorption of CRL onto CLDH. SEM-EDX spectra show CRL presence in CMAN-CRL, CNAN-CRL and CZAN-CRL prepared. Blue spots on the surface of CMAN-CRL, CNAN-CRL and CZAN-CRL stained with Coomasie Blue R confirm protein adsorption onto supports. The catalytic activity of all three CLDH-CRL was also evaluated in synthesis of methyl caprylate and results showed that CMAN-CRL and CNAN-CRL exhibited 70 % conversion while CZAN-CRL exhibited 80 % conversion compared to free lipase that exhibited only 6 % conversion only.

Item Type:Conference or Workshop Item (Paper)
Keyword:Immobilisation; Lipase; Layered double hydroxides; Esterification
Subject:Candida
Subject:Lipase
Subject:Esterification
Faculty or Institute:Faculty of Science
ID Code:8738
Deposited By: Samsida Samsudin
Deposited On:17 Dec 2010 10:51
Last Modified:18 Nov 2014 14:22

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