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Production of Extracellular Protein a from a Methicillin-Resistant Strain of Staphylococcus Aureus

Tahir, Sharifah (1994) Production of Extracellular Protein a from a Methicillin-Resistant Strain of Staphylococcus Aureus. Masters thesis, Universiti Pertanian Malaysia.

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Abstract

Protein A was produced extra cellularly by methicillinresistant Staphylococcus aureus, strain A676. A competitive- ELISA technique based on a competitive pinding between unlabelled protein A and conjugated protein A was employed to measure the concentration of protein A. A study was also conducted to develop this technique. Rabbit IgG was first purified before it was used to coat a microtiterplate. IgG purified using ammonium sulphate precipitation in combination with DEAE-cellulose chromatography gave purer IgG than the caprylic acid method combined with saturated ammonium sulphate or single-step DEAE-cellulose ion exchange chromatography. The molecular weight of the purified IgG was 140,000 dalton based on SDS gelelectrophoresis. The minimum detectable amount of protein A was in the range of 0-20 ng/ml. The optimum concentration of IgG required for coating was 2pg/ml and the incubation time for the substrate was 20 minutes.

Item Type:Thesis (Masters)
Subject:Staphylococcus aureus.
Subject:Proteins.
Subject:Methicillin Resistance.
Chairman Supervisor:Dr. Abdul Rabbi Manaf Ali
Call Number:FSMB 1994 2
Faculty or Institute:Faculty of Food Science and Technology
ID Code:8361
Deposited By: Nurul Hayatie Hashim
Deposited On:12 Nov 2010 09:03
Last Modified:29 Jun 2012 16:56

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