Citric Acid Production by Saccharomycopsis Lipolytica Using Batch Culture: Study on Oxygen Requirement

Osman, Rosmawati (1994) Citric Acid Production by Saccharomycopsis Lipolytica Using Batch Culture: Study on Oxygen Requirement. Masters thesis, Universiti Pertanian Malaysia.

[img] PDF


A citric acid producing yeast strain Saccharomycopsis lipolytic 5054 formerly known as Candida lipolytica was used throughout the study. The strain was obtained from Thailand Institute of Scientific and Technological Research (TISTR). A preliminary study on the development for the optimization of the fermentation process was conducted. The experiments included the effect of medium contents such as CaC03 and initial glucose concentrations, inoculum size, agitation rates, KLa (volumetric oxygen transfer coefficient) and dissolved oxygen concentrations. From the preliminary experiments, Medium I (l0% glucose, 0.1% yeast extract, 0.1% MgS04.7H20, 0.2% NH4CI, 0.1% KH2PO, 1% CaC03, pH 6.6) was selected as the fermentation medium throughout the study. The optimum growth temperature and inoculum size was 30°C and 10%, respectively. 1% CaC03 (w/v) was selected as the optimum concentration. The highest KLa obtained was 29.6 hour-I at the agitation rate of 700 rpm using the Rushton turbine impeller. This impeller speed was employed during the studies on optimum dissolved oxygen concentration, where the highest citric acid production obtained was 11.6 giL during fermentation at 50% (20 mg/L) dissolved oxygen concentration. The optimum fermentation period was 50 hours where the exponential phase ended and the stationary phase started.

Item Type:Thesis (Masters)
Subject:Candida lipolytica
Subject:Citric acid
Chairman Supervisor:Dr. Baharuddin Abdul Ghani
Call Number:FSMB 1994 1
Faculty or Institute:Faculty of Food Science and Technology
ID Code:8360
Deposited By: Nurul Hayatie Hashim
Deposited On:12 Nov 2010 00:58
Last Modified:02 Nov 2012 04:14

Repository Staff Only: Edit item detail

Document Download Statistics

This item has been downloaded for since 12 Nov 2010 00:58.

View statistics for "Citric Acid Production by Saccharomycopsis Lipolytica Using Batch Culture: Study on Oxygen Requirement"

Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.