Sequence analysis and expression of VP3 gene of chicken anaemia virus

M. S., Hasmah and Abdul Rahman, Omar and Ideris, Aini and Bejo, Mohd Hair (2003) Sequence analysis and expression of VP3 gene of chicken anaemia virus. Jurnal Veterinar Malaysia, 15 (1-2). pp. 47-51. ISSN 9128-2506

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Abstract

Chicken anaemia virus (CAV) isolate BL-5 VP3 gene was amplified, sequenced and expressed in E. coli as a fusion protein together with a six-histidine tag. The deduced amino acid sequence of VP3 was identical to other CAV isolates Cux-1, A2 and CIA-1 except for a single amino acid substitution at position 12. SDS-PAGE and Western blot analysis indicated that the expressed protein is insoluble and was found primarily from the cell lysate fraction. Expression of the protein was detected as early as 1 h, with maximum expression (~12% of the total protein) at 6 h post induction with IPTG. This study indicates that VP3 protein is highly expressed in insoluble form in E. coli. However, the biological function of the protein remains to be studied

Item Type:Article
Keyword:amino acid sequences, anaemia, fowl diseases, gene expression, genes, nucleotide sequences, poultry, viral proteins
Faculty or Institute:Faculty of Veterinary Medicine
Publisher:Veterinary Association Malaysia
ID Code:7859
Deposited By: Azizan Arshad
Deposited On:23 Sep 2010 07:04
Last Modified:23 Sep 2010 07:06

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