M. S., Hasmah and Abdul Rahman, Omar and Ideris, Aini and Bejo, Mohd Hair (2003) Sequence analysis and expression of VP3 gene of chicken anaemia virus. Jurnal Veterinar Malaysia, 15 (1-2). pp. 47-51. ISSN 9128-2506
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Chicken anaemia virus (CAV) isolate BL-5 VP3 gene was amplified, sequenced and expressed in E. coli as a fusion protein together with a six-histidine tag. The deduced amino acid sequence of VP3 was identical to other CAV isolates Cux-1, A2 and CIA-1 except for a single amino acid substitution at position 12. SDS-PAGE and Western blot analysis indicated that the expressed protein is insoluble and was found primarily from the cell lysate fraction. Expression of the protein was detected as early as 1 h, with maximum expression (~12% of the total protein) at 6 h post induction with IPTG. This study indicates that VP3 protein is highly expressed in insoluble form in E. coli. However, the biological function of the protein remains to be studied
|Keyword:||amino acid sequences, anaemia, fowl diseases, gene expression, genes, nucleotide sequences, poultry, viral proteins|
|Faculty or Institute:||Faculty of Veterinary Medicine|
|Publisher:||Veterinary Association Malaysia|
|Deposited By:||Azizan Arshad|
|Deposited On:||23 Sep 2010 07:04|
|Last Modified:||23 Sep 2010 07:06|
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