M. S., Hasmah and Abdul Rahman, Omar and Ideris, Aini and Bejo, Mohd Hair (2003) Sequence analysis and expression of VP3 gene of chicken anaemia virus. Jurnal Veterinar Malaysia, 15 (1-2). pp. 47-51. ISSN 9128-2506
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Abstract
Chicken anaemia virus (CAV) isolate BL-5 VP3 gene was amplified, sequenced and expressed in E. coli as a fusion protein together with a six-histidine tag. The deduced amino acid sequence of VP3 was identical to other CAV isolates Cux-1, A2 and CIA-1 except for a single amino acid substitution at position 12. SDS-PAGE and Western blot analysis indicated that the expressed protein is insoluble and was found primarily from the cell lysate fraction. Expression of the protein was detected as early as 1 h, with maximum expression (~12% of the total protein) at 6 h post induction with IPTG. This study indicates that VP3 protein is highly expressed in insoluble form in E. coli. However, the biological function of the protein remains to be studied
| Item Type: | Article |
|---|---|
| Keyword: | amino acid sequences, anaemia, fowl diseases, gene expression, genes, nucleotide sequences, poultry, viral proteins |
| Faculty or Institute: | Faculty of Veterinary Medicine |
| Publisher: | Veterinary Association Malaysia |
| ID Code: | 7859 |
| Deposited By: | Azizan Arshad |
| Deposited On: | 23 Sep 2010 15:04 |
| Last Modified: | 23 Sep 2010 15:06 |
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