Effect of palm oil mill effluent supplementation on cellulase production from rice straw by local fungal isolates

Roslan, Ahmad Muhaimin and Hassan, Mohd Ali and Abdul Aziz, Suraini and Phang, Lai Yee (2009) Effect of palm oil mill effluent supplementation on cellulase production from rice straw by local fungal isolates. International Journal of Agricultural Research, 4 (5). pp. 185-192. ISSN 1816-4897

Full text not available from this repository.

Official URL: http://dx.doi.org/10.3923/ijar.2009.185.192

Abstract

In this study, Palm Oil Mill Effluent (POME) was selected as supplement in rice straw fermentation to replace commercial inducer due to its high concentration of simple carbohydrate and nitrogenous compounds. The cellulase activity of the enzyme produced were then determined individually and combined (enzyme cocktail) to observe increment in cellulase activity. Individually, filter paperase (FPAse), carboxymethyl cellulase (CMCase) and β-glucosidase activities increase for all 3 fungi used except FPAse for Aspergillus sp. (NEW). Highest FPAse and CMCase activities increment observed was from Phanerochaete chrysosporium which were 72 and 236% increment, respectively, while highest β-glucosidase activity was from Aspergillus terreus (AT) which was 298% increment. Meanwhile in cocktail cellulase, synergistic effect was observed especially for FPAse activity whereby highest FPAse activity observed was by cocktail of all the three species of fungi which show 393% increment. Though CMCase increment was observed in this mode, it was not as high as FPAse where the highest CMCase activity observed was cocktail by the three fungal species which increased by 41%. However, in β-glucosidase activity, only cocktail of NEW and AT showed increment from their average activity which was by 6%. It seems that β-glucosidase activity plays major role in determining the total cellulase activity. In the nutshell, supplementation of POME can improve cellulase activity.

Item Type:Article
Keyword:Cellulase, enzyme cocktail, rice straw, fungi, supplement
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences > Environmental Biotechnology Research Group
Publisher:Academic Journals Inc.
DOI Number:10.3923/ijar.2009.185.192
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3923/ijar.2009.185.192
ID Code:7738
Deposited By: Samsida Samsudin
Deposited On:19 Aug 2010 03:47
Last Modified:19 Aug 2010 03:53

Repository Staff Only: item control page

Document Download Statistics

This item has been downloaded for since 19 Aug 2010 03:47.

View statistics for "Effect of palm oil mill effluent supplementation on cellulase production from rice straw by local fungal isolates"


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.