High-Temperature Crystallization of Thermostable T1 Lipase
Thean, Chor Leow and Raja Abdul Rahman, Raja Noor Zaliha and Salleh, Abu Bakar and Basri, Mahiran (2007) High-Temperature Crystallization of Thermostable T1 Lipase. Crystal Growth and Design, 7 (2). pp. 406-410. ISSN 1528-7483
Official URL: http://dx.doi.org/10.1021/cg050506z
The gene encoding thermostable T1 lipase secreted by Geobacillus sp. strain T1 has been overexpressed in a prokaryotic system. Preliminary crystallization was conducted with crystal screen and crystal screen II through a sitting drop vapor diffusion method with 0.5 mg/mL purified T1 lipase at 16 °C. Crystallization at 16 °C using formulation 21 of crystal screen II at 2.5 mg/mL yielded bigger and more defined crystals. Good crystals could easily be obtained as the temperature was increased further while retaining other conditions. In fact, crystallization of T1 lipase is still possible at 60 °C. The ability to form crystals at 60 °C is a new discovery in lipase crystallization.
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