Israf Ali, Daud Ahmad and Tajul Arifin, Khaizurin and Ahmad, Syahida and Lajis, Md Nordin and Shari, Khozirah (2007) Cardamonin inhibits COX and iNOS expression via inhibition of p65NF-κB nuclear translocation and Iκ-B phosphorylation in RAW 264.7 macrophage cells. Molecular Immunology, 44 (5). pp. 673-679. ISSN 0161-5890
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Official URL: http://dx.doi.org/10.1016/j.molimm.2006.04.025
Cardamonin, a chalcone isolated from the fruits of a local plant Alpinia rafflesiana, has demonstrated anti-inflammatory activity in cellular models of inflammation. In this report, we evaluated the ability of cardamonin to suppress both NO and PGE2 synthesis, iNOS and COX-2 expression and enzymatic activity, and key molecules in the NF-κB pathway in order to determine its molecular target. Cardamonin suppressed the production of NO and PGE2 in interferon-γ (IFN-γ)- and lipopolysaccharide (LPS)-induced RAW 264.7 cells. This inhibition was demonstrated to be caused by a dose-dependent down-regulation of both inducible enzymes, iNOS and COX-2, without direct effect upon iNOS or COX-2 enzyme activity. Subsequently we determined that the inhibition of inducible enzyme expression was due to a dose-dependent inhibition of phosphorylation and degradation of I-κBα, which resulted in a reduction of p65NF-κB nuclear translocation. We conclude that cardamonin is a potential anti-inflammatory drug lead that targets the NF-κB pathway.
|Keyword:||Cardamonin, NO,PGE2, iNOS, COX-2, p65NF-κB, I-κBα|
|Faculty or Institute:||Faculty of Science|
|Deposited By:||Najwani Amir Sariffudin|
|Deposited On:||20 Jul 2010 07:06|
|Last Modified:||29 Sep 2010 07:29|
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