Application of polymerase chain reaction assay in detection of Leptospira icterohaemorrhagiae in experimentally infected mice

Omar, Sharina and Bejo, Siti Khairani and Bahaman, Abdul Rani (2007) Application of polymerase chain reaction assay in detection of Leptospira icterohaemorrhagiae in experimentally infected mice. Journal of Animal and Veterinary Advances, 6 (10). pp. 1197-1198. ISSN 1680-5593

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Official URL: http://dx.doi.org/10.3923/javaa.2007.1197.1198

Abstract

A total of 24 mice were inoculated with 1x107 of Leptospira icterohaemorrhagiae. Four mice as negative control were not inoculated. Four inoculated mice were sacrificed at each sampling day on day 1, 3, 7, 10, 14 and 21 post inoculation. Blood and kidney samples were collected and inoculated into JS medium for isolation of L. icterohaemorrhagiae. Polymerase Chain Reaction (PCR) assay was performed to detect the present of the organisms in blood and kidneys. All culture samples were negative for L. icterohaemorrhagiae. However, one (1/24) blood sample was positive by PCR. It is apparent that detection of leptospires in blood with PCR is a promising approach for early diagnosis of leptospirosis.

Item Type:Article
Keyword:blood, detection, diagnosis, diagnostic techniques. experimental infections, kidneys, leptospirosis, polymerase chain reaction
Faculty or Institute:Faculty of Veterinary Medicine
Publisher:Medwell Online
ID Code:7496
Deposited By: Azizan Arshad
Deposited On:16 Jul 2010 04:14
Last Modified:16 Jul 2010 04:17

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