In-house Rose Bengal Plate Agglutination Test (RBPT) for a rapid diagnosis of brucellosis in goats in Malaysia
O., Shahaza and Bejo, Siti Khairani and Zakaria, Zunita and Bahaman, Abdul Rani (2009) In-house Rose Bengal Plate Agglutination Test (RBPT) for a rapid diagnosis of brucellosis in goats in Malaysia. International Journal of Tropical Medicine, 4 (3). pp. 116-118. ISSN 1816-3319
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Official URL: http://dx.doi.org/10.3923/ijtmed.2009.116.118
The Rose Bengal Plate Test (RBPT) antigens from Brucella melitensis local isolates (in-house RBPT) were prepared and compared with RBPT antigen for brucellosis in sheep and goats prepared by Veterinary Laboratory Agency, UK. A total of 856 serum samples collected from goats were examined with the RBPT and Complement Fixation Test (CFT). The RBPT and CFT results showed that the in-house RBPT antigen was superior to the commercial prepared RBPT antigen. Out of 856 sera analysed by in-house RBPT, commercial RBPT and CFT, 30.84, 26.40 and 31.65% were Brucella-positive, respectively. The sensitivity calculated for the in-house RBPT compared with CFT was 85.24%, whilst that of commercial RBPT was 78.59%. Therefore, it is concluded that the in-house RBPT antigens could be prepared and used for epidemiological surveillance of caprine brucellosis in Malaysia
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