Optimisation of enzymatic hydrolysis for concentration of squalene in palm fatty acid distillate

Chua, Camilla Soo Leng and Baharin, Badlishah Sham and Che Man, Yaakob and Tan, Chin Ping (2008) Optimisation of enzymatic hydrolysis for concentration of squalene in palm fatty acid distillate. Journal of the Science of Food and Agriculture, 88 (9). pp. 1512-1517. ISSN 0022-5142

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Official URL: http://dx.doi.org/10.1002/jsfa.3237


BACKGROUND: Squalene was concentrated from palm fatty acid distillate (PFAD) in this study using commercial immobilised Candida antarctica lipase (Novozyme 435®). The PFAD was neutralised (NPFAD) using an alkali to liberate the free fatty acids and then hydrolysed at 65 ± 1 °C. The enzymatic hydrolysis on NPFAD was optimised using response surface methodology (RSM) before being neutralised again to obtain a concentrated squalene fraction. RESULTS: A five-level, three-factor central composite rotatable design was adopted to evaluate the effects of the enzymatic hydrolysis parameters reaction time (4-12 h), water content (50-70% w/w) and enzyme concentration (1.5-3.5% w/w) on the percentage yield of squalene concentration. The optimal reaction parameters for maximum yield of squalene concentration were identified from the respective contour plots. The optimal enzymatic hydrolysis conditions were a reaction time of 7.05 h, a water content of 61.40% w/w and an enzyme concentration of 2.23% w/w. CONCLUSION: RSM was used to determine the optimal conditions for enzymatic hydrolysis of NPFAD with C. antarctica lipase for maximum recovery of squalene which could be implemented on an industrial scale.

Item Type:Article
Keyword:enzymatic hydrolysis, palm fatty acid distillate, Candida antarctica, response surface methodology, squalene
Faculty or Institute:Faculty of Food Science and Technology
Publisher:Wiley Interscience
ID Code:7396
Deposited By: Samsida Samsudin
Deposited On:21 Jun 2010 10:06
Last Modified:13 Jun 2011 08:44

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