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Genetic characterization of the two colour-type of Kelah

Siraj, Siti Shapor and Esa, Yuzine and Keong, Remmy Bun Poh and Daud, Siti Khalijah (2007) Genetic characterization of the two colour-type of Kelah. Malaysian Applied Biololgy, 36 (1). pp. 23-29. ISSN 0126-8643

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Randomly Amplified Polymorphic DNA (RAPD) marker was used to examine the genetic relationships among three populations of two different colour-types (silver-bronze and reddish) of ikan kelah (Tor tambroides). Sixty three individuals of the kelah were sampled from Sia River of Pahang and Kampung Esok River of Negeri Sembilan (silverbronze)and Nenggiri River of Kelantan (reddish). Twelve RAPD primers generated a total of 226 scorable loci with 100% polymorphism across the sixty-three individuals. The RAPD banding patterns and sizes ranged from 4 to 17 and from 100bp to 1500bp, respectively. The intra-population UPGMA dendrogram produced two major clusters, with the Nenggiri River (Kelantan) samples formed a subcluster in both major clusters dominated by the Pahang samples (Cluster 1) and N. Sembilan (Cluster 2) samples, respectively. The inter-population UPGMA showed that the Kelantan samples were genetically closer to the N. Sembilan samples than to the Pahang samples. Thus, the results of this study did not support the hypothesis that the two colour-types of T. tambroides were genetically distinct from each other.

Item Type:Article
Keyword:Freshwater fish, Tor tambroides, colour-type, RAPD, genetic marker
Faculty or Institute:Faculty of Science
Publisher: Malaysian Society of Applied Biology
ID Code:7342
Deposited By: Najwani Amir Sariffudin
Deposited On:16 Jun 2010 12:43
Last Modified:27 May 2013 15:34

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