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Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda)

Seng, Hoi Ling and Ong, Alan Han Kiat and Raja Abdul Rahman, Raja Noor Zaliha and Mohd Yamin, Bohari and Tiekink, Edward R. T. and Tan, Kong Wai and Maah, Mohd Jamil and Caracelli, Ignez and Ng, Chew Hee (2008) Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda). Journal of Inorganic Biochemistry, 102 (11). pp. 1997-2011. ISSN 0162-0134; ESSN: 1873-3344

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Abstract / Synopsis

The binding selectivity of the M(phen)(edda) (M = Cu, Co, Ni, Zn; phen = 1,10-phenanthroline, edda = ethylenediaminediacetic acid) complexes towards ds(CG)6, ds(AT)6 and ds(CGCGAATTCGCG) B-form oligonucleotide duplexes were studied by CD spectroscopy and molecular modeling. The binding mode is intercalation and there is selectivity towards AT-sequence and stacking preference for A/A parallel or diagonal adjacent base steps in their intercalation. The nucleolytic properties of these complexes were investigated and the factors affecting the extent of cleavage were determined to be: concentration of complex, the nature of metal(II) ion, type of buffer, pH of buffer, incubation time, incubation temperature, and the presence of hydrogen peroxide or ascorbic acid as exogenous reagents. The fluorescence property of these complexes and its origin were also investigated. The crystal structure of the Zn(phen)(edda) complex is reported in which the zinc atom displays a distorted trans-N4O2 octahedral geometry; the crystal packing features double layers of complex molecules held together by extensive hydrogen bonding that inter-digitate with adjacent double layers via π…π interactions between 1,10-phenanthroline residues. The structure is compared with that of the recently described copper(II) analogue and, with the latter, included in molecular modeling.

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: 10.1016/j.jinorgbio.2008.07.015
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.jinorgbio.2008.07.015
Publisher: Elsevier
Keywords: Metal(II) ternary complexes; DNA binding; Molecular modeling; Nucleolytic; Intercalation
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 16 Jun 2010 15:03
Last Modified: 26 Apr 2016 16:55
URI: http://psasir.upm.edu.my/id/eprint/7325

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