Enzyme-Catalyzed Production and Chemical Composition of Diacylglycerols from Corn Oil Deodorizer Distillate

Lo, Seong Koon and Baharin, Badlishah Sham and Tan, Chin Ping and Lai, Oi Ming (2005) Enzyme-Catalyzed Production and Chemical Composition of Diacylglycerols from Corn Oil Deodorizer Distillate. Food Biotechnology, 18 (3). pp. 265-278. ISSN 0890-5436

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Official URL: http://dx.doi.org/10.1081/FBT-200035014

Abstract

Diacylglycerols (DAG) were enzymatically synthesized by lipase-catalyzed esterification of glycerol with fatty acids from corn oil deodorizer distillate (CrODD). Effects of reaction parameters such as reaction time, temperature, enzyme type, enzyme load, substrate mole ratio, and water content, as well as the effect of molecular sieves as a water adsorbent were investigated. Rhizomucor miehei lipase (Lipozyme RM IM) was found to be most effective among the lipases screened. The following conditions yielded 70.0% (w/w) DAG: 5 h reaction time, 65°C reaction temperature, 10% (w/w) Lipozyme RM IM, 2.5:1 fatty acid to glycerol molar ratio, and 30% (w/w) molecular sieves. DAG synthesis of 12.4% (w/w) was still observed at 10% (w/w) water content. 84.2% (w/w) of DAG was obtained after purification. The DAG oil comprised predominantly of 1-oleoyl-3-linoleoyl-glycerol (28.5%), 1,3-diolein (22.7%), 1-oleoyl-2-linoleoyl-glycerol (17.9%), and 1,2-diolein (10.9%). Fatty acid profile was similar to that of refined, bleached and deodorised (RBD) corn oil. The ratio of 1,3- to 1,2-positional isomers of DAG was at 1.82:1.

Item Type:Article
Keyword:Corn oil, Deodoriser distillate, Diacylglycerol, Enzymatic synthesis, Esterification, Lipase, Lipozyme RM IM, Rhizomucor miehei
Faculty or Institute:Faculty of Food Science and Technology
Publisher:Taylor & Francis
DOI Number:10.1081/FBT-200035014
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1081/FBT-200035014
ID Code:7280
Deposited By: Erni Suraya Abdul Aziz
Deposited On:15 Jun 2010 08:09
Last Modified:30 Jul 2010 07:28

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