Factors affecting the nucleolytic cleavage of DNA by (N,N′-ethylenendiaminediacetato)metal(II) complexes, M(edda). Crystal structure of Co(edda)

Ng, Chew Hee and Ong, Han Kiat Alan and Kong, Chiak Wu and Tan, Kong Wai and Raja Abdul Rahman, Raja Noor Zaliha and Mohd Yamin, Bohari and Ng, Seik Weng (2006) Factors affecting the nucleolytic cleavage of DNA by (N,N′-ethylenendiaminediacetato)metal(II) complexes, M(edda). Crystal structure of Co(edda). Polyhedron, 25 (16). pp. 3118-3126. ISSN 0277-5387

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Official URL: http://dx.doi.org/10.1016/j.poly.2006.05.020

Abstract

M(edda) complexes (Cu, Co, Ni, Zn) have been characterized by UV–visible spectroscopy and ESI-MS to study the species in aqueous solution. The Co complex crystallizes as the octahedral diaqua(N,N′-ethylenediaminediacetato)cobalt(II) monohydrate and its crystal structure is reported. Cu(edda) cleaves plasmid DNA in the presence of hydrogen peroxide better than Co(edda) while the Zn and Ni analogues are inactive. Partial and complete inhibition of DNA cleavage can be effected by radical scavengers and Na2H2EDTA, respectively. The cleavage efficiency of Cu(edda) varies with the concentration of the complex, pH of the buffer and the type of buffer. The difference in nucleolytic efficiency of Cu(edda) and other complexes studied can be explained by the difference in amount of OH radicals produced, as determined by a PNDA assay. ESI-MS and CD studies on the Cu(edda) complex confirms its binding to DNA and results in a non-denaturational type of conformation change.

Item Type:Article
Keyword:(N,N′-ethylenediaminediacetato)metal(II), DNA cleavage, Crystal structure, Nucleolytic efficiency, Hydrogen peroxide
Faculty or Institute:Faculty of Engineering
Publisher:Elsevier
DOI Number:10.1016/j.poly.2006.05.020
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.poly.2006.05.020
ID Code:7270
Deposited By: Erni Suraya Abdul Aziz
Deposited On:15 Jun 2010 02:10
Last Modified:27 Jul 2010 08:51

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