Descriptive distribution and phylogenetic analysis of feline infectious peritonitis virus isolates of Malaysia

Sharif, Saeed and Arshad, Siti Suri and Bejo, Mohd Hair and Omar, Abdul Rahman and Alauddin, Zeenathul Nazariah and Lau, S. Fong and Rahman, Nor Alimah and Arshad, Habibah and Shamsudin, Shahirudin and Awang Isa, Mohd Kamarudin (2010) Descriptive distribution and phylogenetic analysis of feline infectious peritonitis virus isolates of Malaysia. Acta Veterinaria Scandinavica, 52 (1). ISSN 1751-0147

[img] PDF
1617Kb

Official URL: http://dx.doi.org/10.1186/1751-0147-52-1

Abstract

The descriptive distribution and phylogeny of feline coronaviruses (FCoVs) were studied in cats suspected of having feline infectious peritonitis (FIP) in Malaysia. Ascitic fluids and/or biopsy samples were subjected to a reverse transcription polymerase chain reaction (RT-PCR) targeted for a conserved region of 3'untranslated region (3'UTR) of the FCoV genome. Eighty nine percent of the sampled animals were positive for the presence of FCoV. Among the FCoV positive cats, 80% of cats were males and 64% were below 2 years of age. The FCoV positive cases included 56% domestic short hair (DSH), 40% Persian, and 4% Siamese cats. The nucleotide sequences of 10 selected amplified products from FIP cases were determined. The sequence comparison revealed that the field isolates had 96% homology with a few point mutations. The extent of homology decreased to 93% when compared with reference strains. The overall branching pattern of phylogenetic tree showed two distinct clusters, where all Malaysian isolates fall into one main genetic cluster. These findings provided the first genetic information of FCoV in Malaysia.

Item Type:Article
Faculty or Institute:Faculty of Veterinary Medicine
Publisher:BioMed Central
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1186/1751-0147-52-1
ID Code:7254
Deposited By: Muizzudin Kaspol
Deposited On:14 Jun 2010 02:33
Last Modified:14 Jun 2010 02:33

Repository Staff Only: item control page

Document Download Statistics

This item has been downloaded for since 14 Jun 2010 02:33.

View statistics for "Descriptive distribution and phylogenetic analysis of feline infectious peritonitis virus isolates of Malaysia"


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.