Raja Abdul Rahman, Raja Noor Zaliha and Lee, Poh Geok and Basri, Mahiran and Salleh, Abu Bakar (2006) An organic solvent-stable alkaline protease from Pseudomonas aeruginosa strain K: Enzyme purification and characterization. Enzyme and Microbial Technology, 39 (7). 1484-1491 . ISSN 0141-0229
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Official URL: http://dx.doi.org/10.1016/j.enzmictec.2006.03.038
Abstract
The organic solvent-tolerant strain K protease was purified to homogeneity by ammonium sulphate precipitation and anion exchange chromatography with 124-fold increase in specific activity. The molecular mass of the purified enzyme as revealed by SDS-PAGE electrophoresis is 51,000 Da. The strain K protease was an alkaline metalloprotease with an optimum pH and temperature of 10 and 70 °C, respectively. The enzyme showed stability and activation in the presence of organic solvents with log Pa/w values equal or more than 4.0. After 14 days of incubation, the purified protease was activated 1.11, 1.82, 1.50, 1.75 and 1.80 times in 1-decanol, isooctane, decane, dodecane and hexadecane, respectively.
| Item Type: | Article |
|---|---|
| Keyword: | Pseudomonas, Organic solvent-tolerant bacterium, Characterization, Organic solvent-stable enzyme, Alkaline metaloprotease |
| Faculty or Institute: | Faculty of Biotechnology and Biomolecular Sciences |
| DOI Number: | 10.1016/j.enzmictec.2006.03.038 |
| ID Code: | 7245 |
| Deposited By: | Erni Suraya Abdul Aziz |
| Deposited On: | 14 Jun 2010 09:27 |
| Last Modified: | 27 Jul 2010 16:52 |
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