An organic solvent-stable alkaline protease from Pseudomonas aeruginosa strain K: Enzyme purification and characterization

Raja Abdul Rahman, Raja Noor Zaliha and Lee, Poh Geok and Basri, Mahiran and Salleh, Abu Bakar (2006) An organic solvent-stable alkaline protease from Pseudomonas aeruginosa strain K: Enzyme purification and characterization. Enzyme and Microbial Technology, 39 (7). 1484-1491 . ISSN 0141-0229

Full text not available from this repository.

Official URL:


The organic solvent-tolerant strain K protease was purified to homogeneity by ammonium sulphate precipitation and anion exchange chromatography with 124-fold increase in specific activity. The molecular mass of the purified enzyme as revealed by SDS-PAGE electrophoresis is 51,000 Da. The strain K protease was an alkaline metalloprotease with an optimum pH and temperature of 10 and 70 °C, respectively. The enzyme showed stability and activation in the presence of organic solvents with log Pa/w values equal or more than 4.0. After 14 days of incubation, the purified protease was activated 1.11, 1.82, 1.50, 1.75 and 1.80 times in 1-decanol, isooctane, decane, dodecane and hexadecane, respectively.

Item Type:Article
Keyword:Pseudomonas, Organic solvent-tolerant bacterium, Characterization, Organic solvent-stable enzyme, Alkaline metaloprotease
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
DOI Number:10.1016/j.enzmictec.2006.03.038
ID Code:7245
Deposited By: Erni Suraya Abdul Aziz
Deposited On:14 Jun 2010 01:27
Last Modified:27 Jul 2010 08:52

Repository Staff Only: Edit item detail

Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.