Improved Protection from Velogenic Newcastle Disease Virus Challenge Following Multiple Immunizations With Plasmid DNA Encoding for F And HN Genes

Loke, C. K. and Omar, Abdul Rahman and Abdul Rahim, Raha and Yusoff, Khatijah (2005) Improved Protection from Velogenic Newcastle Disease Virus Challenge Following Multiple Immunizations With Plasmid DNA Encoding for F And HN Genes. Veterinary Immunology and Immunopathology, 106 . pp. 259-267. ISSN 0165-2427

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1016/j.vetimm.2005.03.005

Abstract

Specific-pathogen free (SPF) chicken were inoculated with the plasmid combination and challenged with velogenic NDV. The antibody level against NVD was measured using commercial enzyme linked immunosorbent assay (ELISA). In the first immunization regimen, SPF chickens inoculated twice with NDV-F or NDV-HN constructs elicited antibody responses 1 week after the second injection. However, the levels of the antibody were low and did not confer significant protection from the lethal challenge. In addition, admininistration of the plasmid constructs with Freund’s adjuvant did not improve the level of protection. In the second immunization regimen, chickens inoculsted trice with the plasmid constructs emulsified with Freund”s adjuvant induced significant antibody titers after the third injection. Three out of nine (33.3 %) chickensvaccinated with pEGFP-HN, five of ten (50.0%) chicken vaccinated with pEGFP-F and nine of ten (90.0%) chicken vaccinated with combined pEGFP-F and pEGFP-HN were protected from the challenge. No significant differences in the levels of protection were observed when the chicken were vaccinated with linearized pEGFP-F. the results suggested that more than two injections with both F and HN encoding plasmid DNA were required to induce level of antibodies for protection against velogenic NDV in chickens.

Item Type:Article
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:Elsevier
ID Code:718
Deposited By:INVALID USER
Deposited On:25 Nov 2008 14:12
Last Modified:26 Jul 2010 04:36

Repository Staff Only: item control page


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.